Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | product inhibition forward reaction | Bacillus subtilis | |
UMP | product inhibition, forward reaction | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Bacillus subtilis | |
0.13 | - |
UMP | - |
Bacillus subtilis | |
0.159 | - |
Uracil | - |
Bacillus subtilis | |
1 | - |
diphosphate | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
PyrR protein | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate | ping pong steady state kinetic pattern, ordered bi-bi mechanism, model, no formation of the phosphoribosyl-enzyme intermediate predicted by classic ping pong kinetics | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no catalysis of exchange reaction between uracil-UMP and diphosphate-5-phospho-alpha-D-ribose 1-diphosphate | Bacillus subtilis | ? | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | equilibrium lies far in the direction of UMP formation | Bacillus subtilis | UMP + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | PyrR protein is a bifunctional protein, that primarily regulates the expression of pyrimidine biosynthetic pyr genes, but also catalyses the uracil phosphoribosyltransferase reaction, little amino acid sequence similarities to other bacterial UPRTases | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
UMP | reverse reaction | Bacillus subtilis | |
5.1 | - |
Uracil | forward reaction | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
assay at | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | product inhibition pattern | Bacillus subtilis |