Cloned (Comment) | Organism |
---|---|
cloning from a fetal brain cDNA library, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain M15, phylogenetic analysis, expressionin AD293 cells, tissue and subcellular expression pattern | Homo sapiens |
expressed in Escherichia coli strain M15 and in AD-293 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
calculated from amino acid sequence | Homo sapiens |
38500 | - |
x * 38500, recombinant His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q96BW1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Homo sapiens |
recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | low level of expression | Homo sapiens | - |
heart | - |
Homo sapiens | - |
heart | low level of expression | Homo sapiens | - |
leukocyte | high level of expression | Homo sapiens | - |
leukocyte | high expression level | Homo sapiens | - |
liver | high level of expression | Homo sapiens | - |
liver | high expression level | Homo sapiens | - |
lung | - |
Homo sapiens | - |
lung | low level of expression | Homo sapiens | - |
additional information | quantitative analysis of the tissue expression pattern | Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
prostate gland | low level of expression | Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
skeletal muscle | low level of expression | Homo sapiens | - |
spleen | high level of expression | Homo sapiens | - |
spleen | high expression level | Homo sapiens | - |
thymus | high level of expression | Homo sapiens | - |
thymus | high expression level | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38500, recombinant His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UPRTase | - |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | calculated from amino acid sequence | - |
5.85 |
Homo sapiens | sequence calculation | - |
5.85 |