Cloned (Comment) | Organism |
---|---|
expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
expression of the wild-type enzyme, of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D242A | site-directed mutagenesis, the mutation is responsible for the high increase in activity | Escherichia coli |
D348A | site-directed mutagenesis, the mutation is responsible for the high increase in activity | Escherichia coli |
additional information | construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
additional information | construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli | Escherichia coli |
R100A | site-directed mutagenesis, the mutation is responsible for the high increase in activity | Escherichia coli |
T42M | site-directed mutagenesis, the mutation is responsible for the glyphosate resistance and the low Km for phosphoenolpyruvate | Salmonella enterica subsp. enterica serovar Typhimurium |
T42M | site-directed mutagenesis, the mutation is responsible for the glyphosate resistance and the low Km for phosphoenolpyruvate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | competitive inhibition of the wild-type enzyme, mutant T42M is less sensitive | Escherichia coli | |
glyphosate | competitive inhibition of the wild-type enzyme, mutant T42M is less sensitive | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Escherichia coli | |
0.0018 | - |
phosphoenolpyruvate | pH 7.0, 30°C, mutant StaroA-T42M | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0024 | - |
phosphoenolpyruvate | pH 7.0, 30°C, mutant EcaroA-T42M | Escherichia coli | |
0.0223 | - |
phosphoenolpyruvate | pH 7.0, 30°C, wild-type EcaroA | Escherichia coli | |
0.0436 | - |
phosphoenolpyruvate | pH 7.0, 30°C, wild-type StaroA | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
* 45000, wild-type and mutant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | - |
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0058 | - |
wild-type enzyme, cell exract | Salmonella enterica subsp. enterica serovar Typhimurium |
0.0076 | - |
wild-type enzyme, cell exract | Escherichia coli |
0.0357 | - |
mutant T42M, cell extract | Escherichia coli |
0.136 | - |
mutant T42M, cell extract | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | * 45000, wild-type and mutant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
5-enolpyruvylshikimate-3-phosphate synthase | - |
Escherichia coli |
EcaroA | - |
Escherichia coli |
EPSPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EPSPS | - |
Escherichia coli |
StaroA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
7 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
glyphosate | pH 7.0, 30°C, wild-type StaroA | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0015 | - |
glyphosate | pH 7.0, 30°C, wild-type EcaroA | Escherichia coli | |
0.0128 | - |
glyphosate | pH 7.0, 30°C, mutant StaroA-T42M | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0305 | - |
glyphosate | pH 7.0, 30°C, mutant EcaroA-T42M | Escherichia coli |