Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete reactivation at 3.3 mM but inhibition at 33 mM | Marsilea drummondii | |
cysteine | complete reactivation at 3.3 mM but inhibition at 33 mM | Marsilea drummondii | |
Thioglycollate | greater reactivation at 33 mM than at 3.3 mM | Marsilea drummondii | |
thiosulphate | greater reactivation at 33 mM than at 3.3 mM | Marsilea drummondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 100% inhibition at 1 mM | Marsilea drummondii | |
Cu2+ | 50% of inhibition at 1 mM | Marsilea drummondii | |
Fe2+ | 70% of inhibition at 1 mM | Marsilea drummondii | |
HgCl2 | potent inhibitor at 0.001 mM | Cheilanthes sieberi | |
HgCl2 | potent inhibitor at 0.001 mM | Marsilea drummondii | |
HgCl2 | potent inhibitor at 0.001 mM | Pteridium esculentum | |
HgCl2 | potent inhibitor at 0.001 mM | Velesunio ambiguus | |
Mersalyl acid | potent inhibitor at 0.001 mM | Cheilanthes sieberi | |
Mersalyl acid | potent inhibitor at 0.001 mM | Marsilea drummondii | |
Mersalyl acid | potent inhibitor at 0.001 mM | Pteridium esculentum | |
Mersalyl acid | potent inhibitor at 0.001 mM | Velesunio ambiguus | |
Mn2+ | no inhibition | Marsilea drummondii | |
additional information | not inhibited by EDTA | Marsilea drummondii | |
p-chloromercuribenzoate | complete inhibition at 0.001 mM | Cheilanthes sieberi | |
p-chloromercuribenzoate | complete inhibition at 0.001 mM | Marsilea drummondii | |
p-chloromercuribenzoate | complete inhibition at 0.001 mM | Pteridium esculentum | |
p-chloromercuribenzoate | complete inhibition at 0.001 mM | Velesunio ambiguus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Marsilea drummondii | |
0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Pteridium esculentum | |
0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Cheilanthes sieberi | |
0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Velesunio ambiguus | |
0.5 | - |
beta-picoline | - |
Marsilea drummondii | |
0.7 | - |
Pyridine | - |
Pteridium esculentum | |
0.7 | - |
Pyridine | - |
Velesunio ambiguus | |
0.9 | - |
nicotinic acid | - |
Velesunio ambiguus | |
0.9 | - |
gamma-Picoline | - |
Marsilea drummondii | |
1.4 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Marsilea drummondii | |
1.4 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Pteridium esculentum | |
1.7 | - |
m-phenylene diamine | - |
Marsilea drummondii | |
1.7 | - |
o-phenylene diamine | - |
Marsilea drummondii | |
2 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Velesunio ambiguus | |
2.4 | - |
m-Toluidine | - |
Marsilea drummondii | |
2.5 | - |
Pyridine | - |
Marsilea drummondii | |
2.8 | - |
3-hydroxyaniline | - |
Marsilea drummondii | |
2.9 | - |
3-aminopyridine | - |
Marsilea drummondii | |
3.3 | - |
Quinoline | - |
Marsilea drummondii | |
3.3 | - |
alpha-picoline | - |
Marsilea drummondii | |
3.3 | - |
p-phenylene diamine | - |
Marsilea drummondii | |
3.4 | - |
gamma-picolinic acid | - |
Marsilea drummondii | |
4.2 | - |
2-hydroxyaniline | - |
Marsilea drummondii | |
5 | - |
Pyridine | - |
Cheilanthes sieberi | |
5 | - |
o-mercaptobenzoic acid | best cosubstrate | Marsilea drummondii | |
5.1 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Cheilanthes sieberi | |
6.4 | - |
o-toluidine | - |
Marsilea drummondii | |
9.5 | - |
adenine | - |
Marsilea drummondii | |
12.5 | - |
2-aminopyridine | - |
Marsilea drummondii | |
15.4 | - |
4-aminopyridine | - |
Marsilea drummondii | |
20 | - |
4-Hydroxyaniline | - |
Marsilea drummondii | |
21.2 | - |
alpha-picolinic acid | - |
Marsilea drummondii | |
25 | - |
nicotinic acid | - |
Marsilea drummondii | |
25 | - |
beta-picolinic acid | - |
Marsilea drummondii | |
29 | - |
nicotinic acid | - |
Pteridium esculentum | |
32 | - |
methylamine | - |
Marsilea drummondii | |
33 | - |
L-cysteine | - |
Marsilea drummondii | |
37 | - |
o-aminobenzoic acid | - |
Marsilea drummondii | |
37 | - |
m-aminobenzoic acid | - |
Marsilea drummondii | |
38 | - |
6-aminohexanoic acid | - |
Marsilea drummondii | |
50 | - |
Sulfanilic acid | - |
Marsilea drummondii | |
50 | - |
3-nitroaniline | - |
Marsilea drummondii | |
55 | - |
nicotinic acid | - |
Cheilanthes sieberi | |
77 | - |
sulfanilamide | - |
Marsilea drummondii | |
111 | - |
proline | - |
Marsilea drummondii | |
125 | - |
imidazole | - |
Marsilea drummondii | |
200 | - |
Tris | - |
Marsilea drummondii | |
200 | - |
p-Aminobenzoic acid | - |
Marsilea drummondii | |
400 | - |
hydroxyproline | - |
Marsilea drummondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity | Marsilea drummondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
gel filtration | Pteridium esculentum |
107000 | - |
gel filtration | Cheilanthes sieberi |
110000 | - |
gel filtration | Velesunio ambiguus |
115000 | - |
gel filtration | Marsilea drummondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + adenine | Marsilea drummondii | - |
? | - |
? | |
thiamine + hydroxyproline | Marsilea drummondii | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
thiamine + hydroxyproline | Pteridium esculentum | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
thiamine + proline | Marsilea drummondii | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
thiamine + proline | Pteridium esculentum | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cheilanthes sieberi | - |
rock fern | - |
Marsilea angustifolia | - |
- |
- |
Marsilea drummondii | - |
nardoo | - |
Marsilea mutica | - |
- |
- |
Pteridium esculentum | - |
bracken fern | - |
Velesunio ambiguus | - |
freshwater mussel | - |
Purification (Comment) | Organism |
---|---|
by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column | Marsilea drummondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
frond | extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds | Marsilea drummondii | - |
frond | activity found in lush fronds | Marsilea mutica | - |
frond | activity found in lush fronds | Cheilanthes sieberi | - |
frond | very low activity in lush fronds | Marsilea angustifolia | - |
frond | very low activity in youngs and old fronds | Pteridium esculentum | - |
root | lush growth | Marsilea drummondii | - |
tuber | very low activity | Pteridium esculentum | - |
whole body | activity found | Velesunio ambiguus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + 2-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
thiamine + 2-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine | - |
? | |
thiamine + 2-mercaptobenzoic acid | - |
Marsilea drummondii | ? | - |
? | |
thiamine + 2-methylbenzeneamine | i.e. o-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine | - |
? | |
thiamine + 2-pyridinecarboxylic acid | i.e. alpha-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium | - |
? | |
thiamine + 3-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
thiamine + 3-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine | - |
? | |
thiamine + 3-methylbenzeneamine | i.e. m-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine | - |
? | |
thiamine + 3-nitroaniline | - |
Marsilea drummondii | ? | - |
? | |
thiamine + 3-pyridinecarboxylic acid | i.e. beta-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium | - |
? | |
thiamine + 4-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
thiamine + 4-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine | - |
? | |
thiamine + 4-methylbenzeneamine | i.e. p-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine | - |
? | |
thiamine + 4-pyridinecarboxylic acid | i.e. gamma-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium | - |
? | |
thiamine + 6-aminohexanoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid | - |
? | |
thiamine + adenine | - |
Marsilea drummondii | ? | - |
? | |
thiamine + alpha-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium | - |
? | |
thiamine + aniline | - |
Marsilea drummondii | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + aniline | - |
Pteridium esculentum | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + aniline | - |
Cheilanthes sieberi | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + aniline | - |
Velesunio ambiguus | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + beta-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium | - |
? | |
thiamine + gamma-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium | - |
? | |
thiamine + hydroxyproline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
thiamine + hydroxyproline | - |
Pteridium esculentum | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
thiamine + imidazole | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine | - |
? | |
thiamine + L-cysteine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid | - |
? | |
thiamine + m-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
thiamine + m-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine | - |
? | |
thiamine + methylamine | - |
Marsilea drummondii | ? | - |
? | |
thiamine + nicotinic acid | - |
Pteridium esculentum | ? | - |
? | |
thiamine + nicotinic acid | - |
Cheilanthes sieberi | ? | - |
? | |
thiamine + nicotinic acid | - |
Velesunio ambiguus | ? | - |
? | |
thiamine + o-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
thiamine + o-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine | - |
? | |
thiamine + p-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
thiamine + p-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine | - |
? | |
thiamine + proline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
thiamine + proline | - |
Pteridium esculentum | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
thiamine + pyridine | - |
Marsilea drummondii | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + pyridine | - |
Pteridium esculentum | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + pyridine | - |
Cheilanthes sieberi | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + pyridine | - |
Velesunio ambiguus | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + quinoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium | - |
? | |
thiamine + sulfanilamide | - |
Marsilea drummondii | ? | - |
? | |
thiamine + sulfanilic acid | - |
Marsilea drummondii | ? | - |
? | |
thiamine + Tris | - |
Marsilea drummondii | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Marsilea drummondii |
8 | 9 | - |
Pteridium esculentum |
8 | 9 | - |
Cheilanthes sieberi |
8 | 9 | - |
Velesunio ambiguus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | - |
Marsilea drummondii |
3 | 12 | - |
Pteridium esculentum |
3 | 12 | - |
Cheilanthes sieberi |
3 | 12 | - |
Velesunio ambiguus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | stable in this range | Marsilea drummondii |
3 | 12 | stable in this range | Pteridium esculentum |
3 | 12 | stable in this range | Cheilanthes sieberi |
3 | 12 | stable in this range | Velesunio ambiguus |