Protein Variants | Comment | Organism |
---|---|---|
additional information | RNA interference-based gene silencing of phytoene synthase, the OgPSY RNAi construct driven by the 35S promoter is introduced into protocorm-like body (PLB) of Oncidium orchid by Agrobacterium tumefaciens-mediated transformation | Oncidium hybrid cultivar |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oncidium hybrid cultivar | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncidium hybrid cultivar | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oncidium hybrid cultivar | - |
shoot | - |
Oncidium hybrid cultivar | - |
Synonyms | Comment | Organism |
---|---|---|
PSY | - |
Oncidium hybrid cultivar |
General Information | Comment | Organism |
---|---|---|
malfunction | RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid. PSY-RNAi in transgenic Oncidium alters the expression pattern of biosynthetic pathway and leads to decreased accumulation of carotenoid contents. The transgenic orchids show downregulated level of enzyme PSY and geranyl synthase gene. They display semi-dwarf phenotype, brilliant green leaves, and development-arrested plastids with rare grana. The total carotenoid content is decreased and the efficiency of the photosynthetic electron transport is declined. The chlorophyll level and the expression of chlorophyll biosynthetic genes, such as OgGLUTR and OgCS are dramatically reduced. The endogenous level of gibberellic acid and abscisic acid in the dwarf transformants are 4fold lower than in wild-type plants. Chilling tolerance of the transgenic Oncidium plants is reduced | Oncidium hybrid cultivar |
metabolism | phytoene synthase is the first rate-limiting regulatory enzyme in the carotenoid biosynthesis pathway | Oncidium hybrid cultivar |