Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | preincubation, 1 mM, 30 min prior to assay causes 50% inhibition | Acinetobacter tartarogenes | |
N-ethylmaleimide | preincubation, 1 mM, 30 min prior to assay causes 91% inhibition | Acinetobacter tartarogenes | |
NADH | Ki: 0.006 mM | Acinetobacter tartarogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
putrescine | - |
Acinetobacter tartarogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for full activity, optimal activity at 50 mM | Acinetobacter tartarogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Acinetobacter tartarogenes |
102000 | - |
gel filtration | Acinetobacter tartarogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 putrescine | Acinetobacter tartarogenes | - |
sym-homospermidine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter tartarogenes | - |
ATCC 31105 | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter tartarogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.41 | - |
- |
Acinetobacter tartarogenes |
Storage Stability | Organism |
---|---|
1°C, 20 mM, Tris/HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM putrescine, 0.02% NaN3 | Acinetobacter tartarogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 putrescine | - |
Acinetobacter tartarogenes | sym-homospermidine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Acinetobacter tartarogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Acinetobacter tartarogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter tartarogenes |