Crystallization (Comment) | Organism |
---|---|
native protein and mutant K46A with pyridoxal 5-phosphate and methionine covalently linked as an external aldimine | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H157N | comparable to wild-type | Arabidopsis thaliana |
H157Q | reduced kcat-value, reduced Km-value | Arabidopsis thaliana |
K46A | no enzymic activity, crystallization data | Arabidopsis thaliana |
N77A | reduced kcat-value | Arabidopsis thaliana |
N77D | drastically reduced kcat-value | Arabidopsis thaliana |
Q147A | drastically reduced kcat-value, increased Km-value | Arabidopsis thaliana |
Q147E | drastically reduced kcat-value, increased Km-value | Arabidopsis thaliana |
S269A | reduced kcat-value, reduced Km-value | Arabidopsis thaliana |
S269T | reduced kcat-value, reduced Km-value | Arabidopsis thaliana |
S75A | drastically reduced kcat-value | Arabidopsis thaliana |
S75N | drastically reduced kcat-value | Arabidopsis thaliana |
S75T | drastically reduced kcat-value | Arabidopsis thaliana |
T182A | reduced kcat-value | Arabidopsis thaliana |
T182S | comparable to wild-type | Arabidopsis thaliana |
T185A | drastically reduced kcat-value | Arabidopsis thaliana |
T185S | drastically reduced kcat-value | Arabidopsis thaliana |
T74A | drastically reduced kcat-value | Arabidopsis thaliana |
T74S | reduced kcat-value | Arabidopsis thaliana |
T78A | reduced kcat-value, reduced Km-value | Arabidopsis thaliana |
T78S | reduced kcat-value, reduced Km-value | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
Sulfide | mutant N77D, pH 7.0, 25°C | Arabidopsis thaliana | |
0.21 | - |
Sulfide | mutant S75T, pH 7.0, 25°C | Arabidopsis thaliana | |
0.22 | - |
Sulfide | wild-type, pH 7.0, 25°C | Arabidopsis thaliana | |
0.22 | - |
Sulfide | mutant T182S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.22 | - |
Sulfide | mutant T185A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.23 | - |
Sulfide | mutant N77A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.27 | - |
Sulfide | mutant Q147S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.3 | - |
Sulfide | mutant H157Q, pH 7.0, 25°C | Arabidopsis thaliana | |
0.3 | - |
Sulfide | mutant T78S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.36 | - |
Sulfide | mutant T185S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.39 | - |
Sulfide | mutant T182A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.4 | - |
Sulfide | mutant H157N, pH 7.0, 25°C | Arabidopsis thaliana | |
0.44 | - |
Sulfide | mutant T78A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.54 | - |
Sulfide | mutant S269A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.57 | - |
O3-acetyl-L-serine | mutant S269A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.68 | - |
O3-acetyl-L-serine | mutant T78A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.82 | - |
O3-acetyl-L-serine | mutant T78S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.91 | - |
O3-acetyl-L-serine | mutant H157Q, pH 7.0, 25°C | Arabidopsis thaliana | |
0.94 | - |
O3-acetyl-L-serine | mutant H157N, pH 7.0, 25°C | Arabidopsis thaliana | |
0.96 | - |
O3-acetyl-L-serine | mutant S269T, pH 7.0, 25°C | Arabidopsis thaliana | |
1.2 | - |
O3-acetyl-L-serine | mutant S75T, pH 7.0, 25°C | Arabidopsis thaliana | |
1.2 | - |
O3-acetyl-L-serine | mutant T182A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.4 | - |
O3-acetyl-L-serine | wild-type, pH 7.0, 25°C | Arabidopsis thaliana | |
1.5 | - |
O3-acetyl-L-serine | mutant T185A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.5 | - |
O3-acetyl-L-serine | mutant T74S, pH 7.0, 25°C | Arabidopsis thaliana | |
1.6 | - |
Sulfide | mutant S269T, pH 7.0, 25°C | Arabidopsis thaliana | |
1.6 | - |
O3-acetyl-L-serine | mutant S75A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.6 | - |
O3-acetyl-L-serine | mutant T182S, pH 7.0, 25°C | Arabidopsis thaliana | |
1.6 | - |
O3-acetyl-L-serine | mutant T185S, pH 7.0, 25°C | Arabidopsis thaliana | |
1.6 | - |
O3-acetyl-L-serine | mutant T74A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.7 | - |
Sulfide | mutant Q147A, pH 7.0, 25°C | Arabidopsis thaliana | |
2 | - |
O3-acetyl-L-serine | mutant S75N, pH 7.0, 25°C | Arabidopsis thaliana | |
3.2 | - |
Sulfide | mutant T74S, pH 7.0, 25°C | Arabidopsis thaliana | |
3.8 | - |
O3-acetyl-L-serine | mutant Q147S, pH 7.0, 25°C | Arabidopsis thaliana | |
3.9 | - |
Sulfide | mutant T74A, pH 7.0, 25°C | Arabidopsis thaliana | |
4.4 | - |
O3-acetyl-L-serine | mutant Q147A, pH 7.0, 25°C | Arabidopsis thaliana | |
4.6 | - |
Sulfide | mutant S75A, pH 7.0, 25°C | Arabidopsis thaliana | |
4.7 | - |
O3-acetyl-L-serine | mutant N77A, pH 7.0, 25°C | Arabidopsis thaliana | |
5.7 | - |
Sulfide | mutant S75N, pH 7.0, 25°C | Arabidopsis thaliana | |
10.2 | - |
O3-acetyl-L-serine | mutant N77D, pH 7.0, 25°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O3-acetyl-L-serine + hydrogensulfide | - |
Arabidopsis thaliana | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization data | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
O3-acetyl-L-serine | mutant T74A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.09 | - |
O3-acetyl-L-serine | mutant S75N, pH 7.0, 25°C | Arabidopsis thaliana | |
0.19 | - |
O3-acetyl-L-serine | mutant Q147S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.22 | - |
Sulfide | mutant Q147S, pH 7.0, 25°C | Arabidopsis thaliana | |
0.34 | - |
O3-acetyl-L-serine | mutant N77D, pH 7.0, 25°C | Arabidopsis thaliana | |
0.42 | - |
Sulfide | mutant T74A, pH 7.0, 25°C | Arabidopsis thaliana | |
0.73 | - |
Sulfide | mutant N77D, pH 7.0, 25°C | Arabidopsis thaliana | |
1.07 | - |
O3-acetyl-L-serine | mutant T185A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.44 | - |
Sulfide | mutant T185A, pH 7.0, 25°C | Arabidopsis thaliana | |
1.45 | - |
Sulfide | mutant S75N, pH 7.0, 25°C | Arabidopsis thaliana | |
3.11 | - |
O3-acetyl-L-serine | mutant T185S, pH 7.0, 25°C | Arabidopsis thaliana | |
4.02 | - |
O3-acetyl-L-serine | mutant Q147A, pH 7.0, 25°C | Arabidopsis thaliana | |
5.57 | - |
Sulfide | mutant T185S, pH 7.0, 25°C | Arabidopsis thaliana | |
7.16 | - |
O3-acetyl-L-serine | mutant S75A, pH 7.0, 25°C | Arabidopsis thaliana | |
12.5 | - |
Sulfide | mutant Q147A, pH 7.0, 25°C | Arabidopsis thaliana | |
20.2 | - |
Sulfide | mutant S75A, pH 7.0, 25°C | Arabidopsis thaliana | |
29.5 | - |
Sulfide | mutant S269A, pH 7.0, 25°C | Arabidopsis thaliana | |
34.3 | - |
O3-acetyl-L-serine | mutant S269A, pH 7.0, 25°C | Arabidopsis thaliana | |
51.5 | - |
O3-acetyl-L-serine | mutant S75T, pH 7.0, 25°C | Arabidopsis thaliana | |
82.7 | - |
Sulfide | mutant S75T, pH 7.0, 25°C | Arabidopsis thaliana | |
155 | - |
Sulfide | mutant N77A, pH 7.0, 25°C | Arabidopsis thaliana | |
370 | - |
Sulfide | mutant T78S, pH 7.0, 25°C | Arabidopsis thaliana | |
403 | - |
O3-acetyl-L-serine | mutant N77A, pH 7.0, 25°C | Arabidopsis thaliana | |
456 | - |
O3-acetyl-L-serine | mutant T74S, pH 7.0, 25°C | Arabidopsis thaliana | |
537 | - |
Sulfide | mutant T78A, pH 7.0, 25°C | Arabidopsis thaliana | |
554 | - |
O3-acetyl-L-serine | mutant T78A, pH 7.0, 25°C | Arabidopsis thaliana | |
572 | - |
O3-acetyl-L-serine | mutant S269T, pH 7.0, 25°C | Arabidopsis thaliana | |
775 | - |
O3-acetyl-L-serine | mutant T78S, pH 7.0, 25°C | Arabidopsis thaliana | |
889 | - |
O3-acetyl-L-serine | mutant T182A, pH 7.0, 25°C | Arabidopsis thaliana | |
929 | - |
Sulfide | mutant S269T, pH 7.0, 25°C | Arabidopsis thaliana | |
1055 | - |
O3-acetyl-L-serine | mutant H157Q, pH 7.0, 25°C | Arabidopsis thaliana | |
1520 | - |
O3-acetyl-L-serine | mutant H157N, pH 7.0, 25°C | Arabidopsis thaliana | |
1600 | - |
Sulfide | mutant T182A, pH 7.0, 25°C | Arabidopsis thaliana | |
1660 | - |
O3-acetyl-L-serine | mutant T182S, pH 7.0, 25°C | Arabidopsis thaliana | |
1690 | - |
Sulfide | mutant T182S, pH 7.0, 25°C | Arabidopsis thaliana | |
1700 | - |
Sulfide | mutant T74S, pH 7.0, 25°C | Arabidopsis thaliana | |
1780 | - |
O3-acetyl-L-serine | wild-type, pH 7.0, 25°C | Arabidopsis thaliana | |
1780 | - |
Sulfide | mutant H157Q, pH 7.0, 25°C | Arabidopsis thaliana | |
1990 | - |
Sulfide | mutant H157N, pH 7.0, 25°C | Arabidopsis thaliana | |
2170 | - |
Sulfide | wild-type, pH 7.0, 25°C | Arabidopsis thaliana |