KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
tripolyphosphate | wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.0015 | - |
tripolyphosphate | G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.0016 | - |
tripolyphosphate | G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.003 | - |
tripolyphosphate | wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.0032 | - |
tripolyphosphate | G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.0053 | - |
tripolyphosphate | G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.008 | - |
tripolyphosphate | G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.011 | - |
tripolyphosphate | G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.014 | - |
tripolyphosphate | RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine | Escherichia coli | |
0.015 | - |
tripolyphosphate | G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.024 | - |
tripolyphosphate | G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.026 | - |
tripolyphosphate | RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine | Escherichia coli | |
0.026 | - |
ATP | G6 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.045 | - |
ATP | RLL and G8 mutants, S-adenosylmethionine synthesis | Escherichia coli | |
0.069 | - |
ATP | G7 mutants, S-adenosylmethionine synthesis | Escherichia coli | |
0.073 | - |
ATP | wild-type S-adenosylmethionine synthesis | Escherichia coli | |
0.087 | - |
ATP | G5 mutant S-adenosylmethionine synthesis | Escherichia coli | |
0.092 | - |
L-methionine | wild-type, S-adenosylmethionine synthesis | Escherichia coli | |
0.23 | - |
L-methionine | RLL mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.3 | - |
L-methionine | G6 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.49 | - |
L-methionine | G7 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.62 | - |
L-methionine | G8 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.77 | - |
L-methionine | G5 mutant, S-adenosylmethionine synthesis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A817 | XL1-Blue strain | - |
Escherichia coli XL1-Blue | P0A817 | XL1-Blue strain | - |
Purification (Comment) | Organism |
---|---|
method that includes ammonium sulfate fraction, phenyl-Sepharose HR and hydroxylapetite CHT-1 chromatographies and amminohexyl-Sepharose anion exchange | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Escherichia coli | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | - |
Escherichia coli XL1-Blue | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
tripolyphosphate + H2O | - |
Escherichia coli | diphosphate + phosphate | - |
? | |
tripolyphosphate + H2O | - |
Escherichia coli XL1-Blue | diphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration and native gel electrophoresis | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000217 | - |
tripolyphosphate | G8 mutant, tripolyphosphatase activity | Escherichia coli | |
0.000000367 | - |
S-adenosylmethionine | RLL mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.00000045 | - |
S-adenosylmethionine | G6 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.000000617 | - |
tripolyphosphate | G6 mutant, tripolyphosphatase activity | Escherichia coli | |
0.000000633 | - |
tripolyphosphate | G5 mutant, tripolyphosphatase activity | Escherichia coli | |
0.000000667 | - |
S-adenosylmethionine | G8 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.00000122 | - |
tripolyphosphate | RLL and G7 mutants, tripolyphosphatase activity | Escherichia coli | |
0.00000167 | - |
tripolyphosphate | wild-type, tripolyphosphatase activity | Escherichia coli | |
0.00000217 | - |
S-adenosylmethionine | G5 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.000012 | - |
S-adenosylmethionine | G7 mutant, S-adenosylmethionine synthesis | Escherichia coli | |
0.000417 | - |
S-adenosylmethionine | wild-type, S-adenosylmethionine synthesis | Escherichia coli |