Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D44A | specific activity is only about 2% of that of the wild type enzyme | Rattus norvegicus |
E63A | reduced activity | Rattus norvegicus |
H78L | reduced activity | Rattus norvegicus |
Q200L | mutant lacks the dipyrromethane cofactor and completely loses the catalytic activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
porphobilinogen | wild type enzyme, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) | Rattus norvegicus | |
0.066 | - |
porphobilinogen | mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) | Rattus norvegicus | |
0.24 | - |
porphobilinogen | mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P19356 | - |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap chelating metal affinity column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
mutant enzyme D44A, at 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 year, remains stable | Rattus norvegicus |
4°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 week, without significant change of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | - |
Rattus norvegicus | hydroxylmethylbilane + 4 NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EC 4.3.1.8 | formerly | Rattus norvegicus |
HMB synthase | - |
Rattus norvegicus |
PBG deaminase | - |
Rattus norvegicus |
porphobilinogen deaminase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
only a little loss of activity is found even after 1 h preincubation at 60°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dipyrromethane | - |
Rattus norvegicus |