Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, chromosome mapping at 69.3 min, overexpression in Escherichia coli strain JLM 16 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphonomycin | irreversible inactivation, requires the presence of UDP-GlcNAc | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
phosphoenolpyruvate | - |
Escherichia coli | |
2.5 | - |
UDP-GlcNAc | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44800 | - |
MW deduced from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A749 | gene murZ, at 69.3 min of the chromosome | - |
Purification (Comment) | Organism |
---|---|
native and recombinant from Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.75 | - |
phosphoenolpyruvate | recombinant enzyme | Escherichia coli | |
4.75 | - |
UDP-N-acetyl-D-glucosamine | recombinant enzyme | Escherichia coli | |
4.75 | - |
phosphoenolpyruvate | cosubstrate UDP-N-acetyl-D-glucosamine | Escherichia coli | |
4.75 | - |
UDP-N-acetyl-D-glucosamine | cosubstrate phosphoenolpyruvate | Escherichia coli |