Literature summary for 2.5.1.72 extracted from

Volbeda, A.; Darnault, C.; Renoux, O.; Reichmann, D.; Amara, P.; Ollagnier de Choudens, S.; Fontecilla-Camps, J.C.
Crystal Structures of quinolinate synthase in complex with a substrate analogue, the condensation intermediate, and substrate-derived product (2016), J. Am. Chem. Soc., 138, 11802-11809 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant K219R/Y107F in complex with the first intermediate resulting from the condensation of dihydroxyacetone phosphate with iminoaspartate and the dihydroxyacetone phosphate analogue phosphoglycolohydroxamate, and mutant K219R/Y21F in complex with quinolinic acid. Phosphoglycolohydroxamate binds to NadA with its phosphate group at the site where the carboxylate groups of the first intermediate also bind	Thermotoga maritima

Crystallization (Comment)

Organism

mutant K219R/Y107F in complex with the first intermediate resulting from the condensation of dihydroxyacetone phosphate with iminoaspartate and the dihydroxyacetone phosphate analogue phosphoglycolohydroxamate, and mutant K219R/Y21F in complex with quinolinic acid. Phosphoglycolohydroxamate binds to NadA with its phosphate group at the site where the carboxylate groups of the first intermediate also bind

Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
K219R	mutant is able to bind citrate	Thermotoga maritima
K219R/Y107F	crystallization data. The mutated protein is unable to catalyze the aldo-keto isomerization and/or cyclization of the first intermediate resulting from the condensation of dihydroxyacetone phosphate with iminoaspartate that ultimately leads to quinolinic acid formation	Thermotoga maritima
K219R/Y21F	crystallization data	Thermotoga maritima
Y21F	mutant is able to bind citrate	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X1X7	-	-
Thermotoga maritima DSM 3109	Q9X1X7	-	-

Synonyms

Synonyms	Comment	Organism
NadA	-	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)