Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloro-mercuriphenylsulfonic acid | 0.1 mM, completely inhibits 4HB geranyltransferase activity | Lithospermum erythrorhizon | |
Digitonin | - |
Lithospermum erythrorhizon | |
iodoacetamide | 10 mM, completely inhibits 4HB geranyltransferase activity | Lithospermum erythrorhizon | |
N-Methylmaleimide | 10 mM, completely inhibits 4HB geranyltransferase activity | Lithospermum erythrorhizon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0138 | - |
geranyl diphosphate | pH 7.8, 37°C | Lithospermum erythrorhizon | |
0.0184 | - |
4-hydroxybenzoate | pH 7.8, 37°C | Lithospermum erythrorhizon |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration: 20-50 mM | Lithospermum erythrorhizon |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
341000 | - |
gel filtration | Lithospermum erythrorhizon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | Lithospermum erythrorhizon | regulatory enzyme in the biosynthesis of shikonin | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithospermum erythrorhizon | - |
Sieb. et Zucc. | - |
Purification (Comment) | Organism |
---|---|
Of various detergents examined, digitonin is the most suitable for the solubilization of the enzyme | Lithospermum erythrorhizon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Lithospermum erythrorhizon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.32 | - |
- |
Lithospermum erythrorhizon |
Storage Stability | Organism |
---|---|
-20°C, in the presence of 10% glycerol, frozen enzyme preparations are stable upon storage for at least two months. Protease inhibitors directed against serin-, carboxyl-, and metalloproteases such as PMSF, 1,2-epoxy-3-(4-nitrophenoxy)-propane or EGTA do not increase the stability of the enzyme solution. | Lithospermum erythrorhizon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | regulatory enzyme in the biosynthesis of shikonin | Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? | |
geranyl diphosphate + 4-hydroxybenzoate | the enzyme is highly specific for geranyl diphosphate and 4-hydroxybenzoate | Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4HB geranyltransferase | - |
Lithospermum erythrorhizon |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
- |
Lithospermum erythrorhizon |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 63 | half-maximal activity at 35°C and at 63°C | Lithospermum erythrorhizon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
in Bis-Tris-HCl buffer | Lithospermum erythrorhizon |
8.5 | - |
in Tris-HCl buffer | Lithospermum erythrorhizon |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9.3 | half-maximal activity at pH 6.2 and at pH 9.3, Tris-HCl buffer | Lithospermum erythrorhizon |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
at pH 4 or 8, all activtiy is lost quickly | Lithospermum erythrorhizon |
5 | - |
storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days | Lithospermum erythrorhizon |
6 | - |
at pH 6, the enzyme can be stored in the presence of 1.5 mM digitonin, 10% glycerol and 2 mM DTT at 4°C for two weeks with minimal loss of activity | Lithospermum erythrorhizon |
7 | - |
storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days | Lithospermum erythrorhizon |
8 | - |
at pH 4 or 8, all activtiy is lost quickly | Lithospermum erythrorhizon |
General Information | Comment | Organism |
---|---|---|
physiological function | regulatory enzyme in the biosynthesis of shikonin | Lithospermum erythrorhizon |