Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion, crystals of mutants P195A and P138A/P195A in complex with maleate, protein solution: 20 mg/ml, 10 mM potassium phosphate, 0.01 mM pyridoxal 5'-phosphate, 2 mM EDTA, 0.5 mM DTT, reservoir solution: 1.9-2.1 M ammonium sulfate, 0.2 M 2-methylmorpholine, 2% polyethylene glycol 400, mutant P138A/P195A is also crystallized in complex with maleate, 20 mM, X-ray diffraction structure analysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
P138A | oligonucleotide-directed mutagenesis, no significant effect, unaltered compared to the wild-type | Escherichia coli |
P138A/P195A | oligonucleotide-directed mutagenesis | Escherichia coli |
P195A | oligonucleotide-directed mutagenesis, no significant effect, unaltered compared to the wild-type | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00509 | - |
- |
Renatured (Comment) | Organism |
---|---|
wild-type and mutants, unfolding by guanidinium hydrochloride and renaturing kinetics, fluorescent spectrometric analysis | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AspAT | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |