Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | 0.5 mM, 14% inhibition | Bacillus cereus | |
glutathione | 0.5 mM, 12% inhibition | Bacillus cereus | |
Monoiodoacetic acid | 0.5 mM, 24% inhibition | Bacillus cereus | |
p-chloromercuribenzoate | 0.5 mM, 71% inhibition | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
beta-Alanine | pH 10.0, 35°C, cosubstrate pyruvate | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
K-22 | - |
Bacillus cereus K-22 | - |
K-22 | - |
Purification (Comment) | Organism |
---|---|
K-22, partial purification | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-alanine + 2-oxobutanoate | 2-oxo-n-butyric acid | Bacillus cereus | malonic semialdehyde + 2-aminobutanoate | - |
? | |
beta-alanine + 2-oxobutanoate | at 64% of the activity with pyruvate | Bacillus cereus | malonic semialdehyde + 2-aminobutanoate | - |
? | |
beta-alanine + 2-oxobutanoate | 2-oxo-n-butyric acid | Bacillus cereus K-22 | malonic semialdehyde + 2-aminobutanoate | - |
? | |
beta-alanine + 2-oxoisopentanoate | at 13% of the activity with pyruvate | Bacillus cereus | malonic semialdehyde + L-valine | - |
? | |
beta-alanine + 2-oxoisopentanoate | at 13% of the activity with pyruvate | Bacillus cereus K-22 | malonic semialdehyde + L-valine | - |
? | |
beta-alanine + 2-oxopentanoate | at 27% of the activity with pyruvate | Bacillus cereus | malonic semialdehyde + L-norvaline | - |
? | |
beta-alanine + 2-oxopentanoate | at 27% of the activity with pyruvate | Bacillus cereus K-22 | malonic semialdehyde + L-norvaline | - |
? | |
beta-alanine + oxaloacetic acid | at 115% of the activity with pyruvate | Bacillus cereus | malonic semialdehyde + L-aspartate | - |
? | |
beta-alanine + oxaloacetic acid | at 115% of the activity with pyruvate | Bacillus cereus K-22 | malonic semialdehyde + L-aspartate | - |
? | |
beta-alanine + pyruvate | i.e. 3-aminopropanoate + 2-oxopropanoate | Bacillus cereus | malonic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | i.e. 3-aminopropanoate + 2-oxopropanoate | Bacillus cereus K-22 | malonic semialdehyde + L-alanine | - |
? | |
pyruvate + 2-aminoethylphosphonic acid | at 28% of the activity with beta-alanine | Bacillus cereus | L-alanine + 2-oxoethylphosphonic acid | - |
? | |
pyruvate + 4-aminobutanoate | at 43% of the activity with beta-alanine | Bacillus cereus | L-alanine + succinic semialdehyde | - |
? | |
pyruvate + 6-aminohexanoate | at 9% of the activity with beta-alanine | Bacillus cereus | L-alanine + 6-oxohexanoate | - |
? | |
pyruvate + propylamine | at 37% of the activity with beta-alanine | Bacillus cereus | L-alanine + propanal | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | stable | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein, 40% stimulation on addition of the cofactor even after the enzyme has previously been incubated with saturating amounts of pyridoxal phosphate | Bacillus cereus |