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Literature summary for 2.6.1.19 extracted from

  • Richter, A.A.; Mais, C.N.; Czech, L.; Geyer, K.; Hoeppner, A.; Smits, S.H.J.; Erb, T.J.; Bange, G.; Bremer, E.
    Biosynthesis of the stress-protectant and chemical chaperon ectoine biochemistry of the transaminase EctB (2019), Front. Microbiol., 10, 2811 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.19

Cloned(Commentary)

Cloned (Comment) Organism
gene ectB, recombinant overexpression of codon-optimized version of (Pl)ectB as N-terminally Strep II-tagged protein from modified plasmid pLC52 in Escherichia coli strain BL21 and TOP10 cells Paenibacillus lautus

Protein Variants

Protein Variants Comment Organism
K274A site-directed mutagenesis, the mutant is unable to bind cofactor PLP and is catalytically inactive Paenibacillus lautus
K274H site-directed mutagenesis, the mutant is unable to bind cofactor PLP and is catalytically inactive. This amino acid substitution does not affect the quaternary assembly of the mutant protein Paenibacillus lautus
K274R site-directed mutagenesis, the mutant is unable to bind cofactor PLP and is catalytically inactive Paenibacillus lautus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Paenibacillus lautus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 about, recombinant Strep II-tagged enzyme, gel filtration Paenibacillus lautus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate Paenibacillus lautus
-
 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate Paenibacillus lautus Y412MC10
-
 succinate semialdehyde + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus lautus D3EKC0 cf. EC 2.6.1.76; isolated from the effluent of a hot spring in the Yellowstone National Park (United States)
-
Paenibacillus lautus Y412MC10 D3EKC0 cf. EC 2.6.1.76; isolated from the effluent of a hot spring in the Yellowstone National Park (United States)
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep II-tagged enzyme from Escherichia coli by streptavidin-affinity chromatography Paenibacillus lautus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture growth at temperatures of up to 50°C Paenibacillus lautus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate
-
 Paenibacillus lautus succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate
-
 Paenibacillus lautus Y412MC10 succinate semialdehyde + L-glutamate
-
 ?
additional information (Pl)EctB also preferably catalyzes a EctB-type reaction, EC 2.6.1.76 Paenibacillus lautus ?
-
-
additional information (Pl)EctB also preferably catalyzes a EctB-type reaction, EC 2.6.1.76 Paenibacillus lautus Y412MC10 ?
-
-

Subunits

Subunits Comment Organism
homotetramer 4 * 49100, recombinant Strep II-tagged enzyme, SDS-PAGE Paenibacillus lautus

Synonyms

Synonyms Comment Organism
GABA-TA
-
 Paenibacillus lautus
More see also EC 2.6.1.76 Paenibacillus lautus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP, residue Lys274 attaches the PLP cofactor to enzyme (Pl)EctB. The architecture of the PLP-binding site, formed by two monomeric subunits, overview Paenibacillus lautus

General Information

General Information Comment Organism
evolution EctB-type proteins are highly conserved Paenibacillus lautus
additional information structural homology modeling of (Pl)EctB usimg the crystal structure of the GABA transaminase from Arthrobacter aurescens (PDB ID 4ATP, EC 2.6.1.19) as template Paenibacillus lautus