Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | complete inhibition at 0.5 mM | Escherichia coli | |
hydroxylamine | complete inhibition at 0.5 mM | Escherichia coli | |
Semicarbazide | complete inhibition at 0.5 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
2-oxoglutarate | pH 7.6, room temperature | Escherichia coli | |
2.7 | - |
pyruvate | pH 7.6, room temperature | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + 2-oxoglutarate | Escherichia coli | - |
4-aminobutanal + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
precipitation with ammonium sulphate followed by column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.63 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-15ºC, 0.03 M Tris-HCl buffer, pH 7.6, 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-1,4-diaminobutan-2-ol + 2-oxoglutarate | 30% of the activity with putrescine | Escherichia coli | ? | - |
? | |
1,6-diaminohexane + 2-oxoglutarate | - |
Escherichia coli | 6-aminohexanal + L-glutamate | - |
? | |
1,7-diaminoheptane + 2-oxoglutarate | 30% of the activity with putrescine | Escherichia coli | 7-aminoheptanal + L-glutamate | - |
? | |
4-aminobutanoate + 2-oxoglutarate | - |
Escherichia coli | 4-oxobutanoate + L-glutamate | - |
? | |
cadaverine + 2-oxoglutarate | equally active as putrescine | Escherichia coli | 5-aminopentanal + L-glutamate | - |
? | |
additional information | inactive with 1,3-diaminopropane, lysine, ornithine, spermidine | Escherichia coli | ? | - |
? | |
additional information | inactive with histamine, tyramine, 4-aminobutanol, beta-alanine | Escherichia coli | ? | - |
? | |
putrescine + 2-oxoglutarate | - |
Escherichia coli | 4-aminobutanal + L-glutamate | - |
? | |
putrescine + pyruvate | - |
Escherichia coli | 4-aminobutanal + L-alanine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | purified enzyme normally active without added pyridoxal phosphate, absolute requirement when irradiated with UV light or treated with 2 mM phenylhydrazine and then with Norite | Escherichia coli |