Any feedback?
Literature summary for 2.6.1.33 extracted from
- Characterization and investigation of substrate specificity of the sugar aminotransferase WecE from E. coli K12 (2004), Chem. Biol., 11, 915-925.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene WecC, amino acid sequence determination and analysis, overexpression of C-terminally His6-tagged enzyme in strain BL21(DE3), phylogenetic analysis | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (aminooxy)acetic acid | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli |  |
| gabaculine | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli | |
| hydroxylamine | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics in a coupled assay | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
| 44452 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
| 44465 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
| 180000 | - |
recombinant His6-tagged enzyme, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene WecC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from strain BL21(DE3) by nickel chelate and hydrophobic interaction chromatography | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dTDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate | substrate specificity might be related to conserved motifs VLalpha, VLbeta, and VLgamma, around the active site | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity measurement in a coupled assay with NADH-dependent L-GDH | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate | - |
? | |
| dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-6-deoxy-D-mannose + 2-oxoglutarate | - |
? | |
| additional information | no activity with dGDP-4-keto-6-deoxy-D-mannose, the nucleotide moiety is a key determinant for acceptor substrate specificity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAT | - |
Escherichia coli |
| sugar aminotransferase | - |
Escherichia coli |
| WecE | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | 25% of maximal activity at 45°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | enzyme shows over 80% of maximal activity at pH 6.0 and pH 8.0, and very low activity below pH 5.0 and above pH 9.5 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
