Application | Comment | Organism |
---|---|---|
biotechnology | production of beta-lactam antibiotics | Actinomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48810 | - |
lysine epsilon-aminotransferase is a 450 amino acid protein | Actinomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | Actinomyces sp. | - |
2-aminoadipate 6-semialdehyde + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
lysine epsilon-aminotransferase is partially purified from a high-producing strain of Streptomyces lactamdurans | Actinomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
lysine epsilon-aminotransferase activity peaks earlier than cephamycin formation in producer strains and an increase in lat copy number results in a 2- to 5fold increase of cephamycin formation, suggesting that formation of alpha-aminoadipic acid is a limiting step for cephamycin formation | Actinomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | - |
Actinomyces sp. | 2-aminoadipate 6-semialdehyde + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAT | - |
Actinomyces sp. |
lysine epsilon-aminotransferase | - |
Actinomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | lysine epsilon-aminotransferase requires pyridoxal 5'-phosphate, which has a binding site at Lys300 of the Nocardia lactamdurans LAT protein as cofactor | Actinomyces sp. |