Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 10 mM, complete inhibition | Thermococcus litoralis | |
L-Penicillamine | 10 mM, 86% inhibition | Thermococcus litoralis | |
additional information | not inhibitory: 10 mM EDTA, 10 mM p-chloromercuribenzoate | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
glyoxylate | - |
Thermococcus litoralis | |
0.92 | - |
L-alanine | - |
Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
45169 | - |
4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
170000 | - |
PAGE | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29 | - |
37°C, pH 7.5 | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | - |
Thermococcus litoralis | glycine + pyruvate | - |
? | |
additional information | strict specificity for L-alanine and glyoxylate as substrates | Thermococcus litoralis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | N-terminal animo acid sequence | Thermococcus litoralis |
tetramer | 4 * 42000, SDS-PAGE, 4 * 45169, claculated | Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
activity increases with temperature from 37°C to 90°C | Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, stabel up to | Thermococcus litoralis |
90 | - |
10 min, less than 50% residual activity | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermococcus litoralis |