Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-glutamate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
2-oxoglutarate | pH 7.6, 37°C, enzyme form I | Rattus norvegicus | |
1 | - |
2-oxoglutarate | pH 7.6, 37°C, enzyme form III | Rattus norvegicus | |
1.1 | - |
2-oxoglutarate | pH 7.6, 37°C, enzyme form II | Rattus norvegicus | |
1.2 | - |
2-oxoglutarate | pH 7.6, 37°C, enzyme form IV | Rattus norvegicus | |
1.4 | - |
L-tyrosine | pH 7.6, 37°C, enzyme form II | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
gel filtration, different isoenzymes | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
4 enzyme forms from kidney, heart and brain | - |
Purification (Comment) | Organism |
---|---|
separation of 4 enzyme forms: I, II, III, IV, using hydroxyapatite chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
effect of glucagon, cyclic nucletides, insulin and hydrocortisone on activity | Rattus norvegicus |
additional information | - |
activities of different isoenzymes from liver, kidney and heart | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | - |
Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
tryptophan + 2-oxoglutarate | - |
Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxamine 5'-phosphate | can replace pyridoxal 5'-phosphate | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
20.3 | - |
L-glutamate | pH 7.6, 37°C, isozyme I from kidney, L-tyrosine as substrate | Rattus norvegicus | |
26.8 | - |
L-glutamate | pH 7.6, 37°C, isozyme IV from liver, L-tyrosine as substrate | Rattus norvegicus | |
27.4 | - |
L-glutamate | pH 7.6, 37°C, isozyme III from liver, L-tyrosine as substrate | Rattus norvegicus | |
31.3 | - |
L-glutamate | pH 7.6, 37°C, isozyme II from liver, L-tyrosine as substrate | Rattus norvegicus |