Cloned (Comment) | Organism |
---|---|
sequence comparisons and phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli by using a cold-shock promotor | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.88 | - |
L-glutamate | pH 8.0, 25°C, recombinant enzyme | Bombyx mori | |
6 | - |
3-phosphooxypyruvate | pH 8.0, 25°C, recombinant enzyme | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphooxypyruvate + L-glutamate | Bombyx mori | - |
O-phospho-L-serine + 2-oxoglutarate | - |
r | |
3-phosphooxypyruvate + L-glutamate | Bombyx mori p50T | - |
O-phospho-L-serine + 2-oxoglutarate | - |
r | |
4-phosphooxy-L-threonine + 2-oxoglutarate | Bombyx mori | - |
(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate | - |
r | |
4-phosphooxy-L-threonine + 2-oxoglutarate | Bombyx mori p50T | - |
(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate | - |
r | |
O-phospho-L-serine + 2-oxoglutarate | Bombyx mori | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
O-phospho-L-serine + 2-oxoglutarate | Bombyx mori b94 | - |
3-phosphooxypyruvate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | H9JSH4 | - |
- |
Bombyx mori | Q2F5M8 | - |
- |
Bombyx mori b94 | Q2F5M8 | - |
- |
Bombyx mori p50T | H9JSH4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant SUMO-His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, to homogeneity | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | high expression level | Bombyx mori | - |
hemocyte | low expression level | Bombyx mori | - |
larva | - |
Bombyx mori | - |
midgut | high expression level | Bombyx mori | - |
additional information | the enzyme activity in strain p50T is higher compared to strain b94 | Bombyx mori | - |
ovary | moderate expression level | Bombyx mori | - |
silk gland | middle silk gland, high expression level | Bombyx mori | - |
testis | moderate expression level | Bombyx mori | - |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, pH 2.0-12.0, 24 h, over 80% activity remaining | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphooxypyruvate + L-glutamate | - |
Bombyx mori | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
3-phosphooxypyruvate + L-glutamate | - |
Bombyx mori p50T | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
4-phosphooxy-L-threonine + 2-oxoglutarate | - |
Bombyx mori | (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate | - |
r | |
4-phosphooxy-L-threonine + 2-oxoglutarate | - |
Bombyx mori p50T | (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate | - |
r | |
additional information | the enzyme preferentially utilizes glutamate for transamination | Bombyx mori | ? | - |
- |
|
additional information | the enzyme preferentially utilizes glutamate for transamination | Bombyx mori p50T | ? | - |
- |
|
additional information | the enzyme preferentially utilizes glutamate for transamination | Bombyx mori b94 | ? | - |
- |
|
O-phospho-L-serine + 2-oxoglutarate | - |
Bombyx mori | 3-phosphooxypyruvate + L-glutamate | - |
r | |
O-phospho-L-serine + 2-oxoglutarate | - |
Bombyx mori b94 | 3-phosphooxypyruvate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40199, sequence calculation, x * 66000, recombinant SUMO-His6-tagged enzyme, SDS-PAGE | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
bmPSAT | - |
Bombyx mori |
phosphoserine aminotransferase | - |
Bombyx mori |
PSAT | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bombyx mori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | 80% of maximal activity within this range | Bombyx mori |
4 | 10 | 50% of maximal activity within this range | Bombyx mori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | 4°C, purified recombinant enzyme, 24 h, over 80% activity remaining | Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP | Bombyx mori |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bombyx mori | sequence calculation | - |
6.97 |
General Information | Comment | Organism |
---|---|---|
malfunction | a sericin-deficient silkworm strain exhibits a diminished expression of bmPSAT mRNA in the silk gland | Bombyx mori |
metabolism | phosphoserine aminotransferase (bmPSAT) from Bombyx mori is responsible for L-serine biosynthesis. This pathway composed of D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), PSAT, and phosphoserine phosphatase (PSP, EC 3.1.3.3) is crucial for the de novo production of L-serine from a glycolytic intermediate, 3-phosphoglycerate | Bombyx mori |
physiological function | phosphoserine aminotransferase (bmPSAT) from Bombyx mori is responsible for L-serine biosynthesis. bmPSAT may play an important role in synthesizing and supplying L-serine in the larva of Bombyx mori. The silk gland is the sole organ in which silk is synthesized and secreted in the silkworm. Silk is composed of two types of proteins: sericin and fibroin, both of which contain high levels of L-serine and glycine | Bombyx mori |