Cloned (Comment) | Organism |
---|---|
gene PSAT1 | Arabidopsis thaliana |
gene PSAT2 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PSAT1-silenced lines (ts-psat1.1 and ts-psat1.2) are generated for functional characterization using a microRNA-based approach. Overexpression of the artificial PSAT1-silencing construct in Arabidopsis thaliana leads to a significant reduction of PSAT1 expression, which subsequently results in a strong inhibition of growth. The expression of the PSAT2 gene is unaltered in PSAT1-silenced plants, phenotype, overview | Arabidopsis thaliana |
additional information | PSAT1-silenced lines (ts-psat1.1 and ts-psat1.2) are generated for functional characterization using a microRNA-based approach. The expression of the PSAT2 gene is unaltered in PSAT1-silenced plants, phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-serine + 2-oxoglutarate | Arabidopsis thaliana | - |
3-phosphooxypyruvate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q96255 | - |
- |
Arabidopsis thaliana | Q9SHP0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | PSAT1 is the most expressed isoform and the expression of both genes is higher in root than in shoot tissue. In 10-days-old plants the activity of both PSAT gene promoters is high in the shoot apical meristem (SAM), the root apical meristem (RAM), in the vasculature of the hypocotyl and in the vasculature of roots, whereas the PSAT1 promoter is additionally active in the vasculature tissue of cotyledons and young leaves. Specific expression pattern of PSAT genes, overview | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-serine + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-phosphooxypyruvate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
phosphoserine aminotransferase1 | - |
Arabidopsis thaliana |
phosphoserine aminotransferase2 | - |
Arabidopsis thaliana |
PSAT1 | - |
Arabidopsis thaliana |
PSAT2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the Arabidopsis genome contains three genes for the PGDH (At4g34200/PGDH1, At1g17745/ PGDH2, and At3g19480/ PGDH3), two genes for the PSAT (At4g35630/PSAT1, and At2g17630/PSAT2) and one gene for the PSP (At1g18640). The PSAT1 gene is the most expressed isoform in Arabidopsis thaliana | Arabidopsis thaliana |
malfunction | a growth phenotype is observed for PSAT1-silenced plants due to serine deficiency | Arabidopsis thaliana |
metabolism | the serine biosynthesis pathway consists of three sequential reactions that are catalyzed by 3-phosphoglycerate dehydrogenase (PGDH), 3-phosphoserine aminotransferase (PSAT), and 3-phosphoserine phosphatase (PSP) enzymes, all localized in the plastids. Serine biosynthesis pathways in plants, overview | Arabidopsis thaliana |
physiological function | phosphoserine aminotransferase1 is part of the phosphorylated pathways for serine biosynthesis and essential for light and sugar-dependent growth promotion | Arabidopsis thaliana |