Cloned (Comment) | Organism |
---|---|
transient expression in HEK-293 cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
2-oxoglutarate | pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine | Rattus norvegicus | |
0.95 | - |
L-kynurenine | pH 8.0, 37°C, recombinant enzyme | Rattus norvegicus | |
1.36 | - |
L-3-hydroxykynurenine | pH 8.0, 37°C, recombinant enzyme | Rattus norvegicus | |
5.6 | - |
L-glutamate | pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Rattus norvegicus |
90000 | 100000 | gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q64602 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, phenyl-Sepharose, Mono Q, Mono P | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.56 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3-hydroxykynurenine + 2-oxoglutarate | - |
Rattus norvegicus | xanthurenic acid + L-glutamate | - |
? | |
L-kynurenine + 2-oxoadipate | 91% of activity with 2-oxoglutarate | Rattus norvegicus | kynurenic acid + 2-aminoadipate + H2O | - |
? | |
L-kynurenine + 2-oxobutyrate | 28% of activity with 2-oxoglutarate | Rattus norvegicus | kynurenic acid + 2-aminobutanoate + H2O | - |
? | |
L-kynurenine + 2-oxoglutarate | enzyme has both kynurenine aminotransferase and alpha-aminoadipate aminotransferase activity | Rattus norvegicus | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + 4-methyl-2-oxovalerate | 5.9% of activity with 2-oxoglutarate | Rattus norvegicus | kynurenic acid + 2,4-dimethylvalerate + H2O | - |
? | |
L-kynurenine + phenylpyruvate | 43% of activity with 2-oxoglutarate | Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine | - |
? | |
L-kynurenine + pyruvate | 3% of activity with 2-oxoglutarate | Rattus norvegicus | kynurenic acid + L-alanine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
KAT | - |
Rattus norvegicus |