Crystallization (Comment) | Organism |
---|---|
KAT II complex with 2-oxoglutarate, hanging drop vapor diffusion method | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxykynurenine | less than 35% inhibition at 5 mM | Homo sapiens | |
aminoadipate | less than 35% inhibition at 5 mM | Homo sapiens | |
L-asparagine | less than 35% inhibition at 5 mM | Homo sapiens | |
L-cysteine | less than 35% inhibition at 5 mM | Homo sapiens | |
L-glutamate | less than 35% inhibition at 5 mM | Homo sapiens | |
L-histidine | less than 35% inhibition at 5 mM | Homo sapiens | |
L-lysine | less than 35% inhibition at 5 mM | Homo sapiens | |
L-phenylalanine | less than 35% inhibition at 5 mM | Homo sapiens | |
additional information | not inhibited by glyoxylate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
2-oxoglutarate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.4 | - |
indo-3-pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.5 | - |
2-oxocaproate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.5 | - |
hydroxyphenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.8 | - |
phenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
2.4 | - |
2-oxo-4-methylthiobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
2.8 | - |
Mercaptopyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.3 | - |
4-methyl-2-oxopentanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.4 | - |
2-oxovalerate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
4.7 | - |
L-kynurenine | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
9.7 | - |
pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
12.7 | - |
2-oxobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
12.9 | - |
3-methyl-2-oxobutanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
14.2 | - |
3-methyl-2-oxobutanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
16.8 | - |
oxaloacetate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
18 | - |
glyoxylate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
20.9 | - |
2-oxoadipate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N5Z0 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and gel filtration | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, isolated KAT II, no noticeable decrease in specific activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + 2-oxo-4-methylthiobutyrate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 2-oxoadipate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 2-oxobutyrate | - |
Homo sapiens | kynurenic acid + 2-aminobutanoate + H2O | - |
? | |
L-kynurenine + 2-oxocaproate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 2-oxoglutarate | - |
Homo sapiens | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + 2-oxovalerate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 3-methyl-2-oxobutanoate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 3-methyl-2-oxopentanoate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + 4-methyl-2-oxopentanoate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + glyoxylate | glyoxylate shows a relatively low affinity for KAT II | Homo sapiens | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + hydroxyphenylpyruvate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + indo-3-pyruvate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + mercaptopyruvate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + oxaloacetate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + phenylpyruvate | - |
Homo sapiens | ? | - |
? | |
L-kynurenine + pyruvate | - |
Homo sapiens | ? | - |
? | |
additional information | 2-oxoglutarate, 2-oxocaproate, phenylpyruvate, and 2-oxo-4-methylthiobutyrate are highly efficient as amino-group acceptors for KAT II | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KAT II | - |
Homo sapiens |
kynurenine aminotransferase II | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
oxaloacetate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.36 | - |
pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.39 | - |
hydroxyphenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.92 | - |
3-methyl-2-oxobutanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.92 | - |
3-methyl-2-oxopentanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.49 | - |
indo-3-pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
2.51 | - |
4-methyl-2-oxopentanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
2.66 | - |
2-oxovalerate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.49 | - |
2-oxobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.59 | - |
Mercaptopyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.64 | - |
glyoxylate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
4.27 | - |
2-oxo-4-methylthiobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
4.73 | - |
phenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
4.83 | - |
2-oxocaproate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
4.84 | - |
2-oxoadipate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
7.67 | - |
2-oxoglutarate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
9.76 | - |
L-kynurenine | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.065 | - |
3-methyl-2-oxopentanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.07 | - |
3-methyl-2-oxobutanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.09 | - |
oxaloacetate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.2 | - |
glyoxylate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.23 | - |
2-oxoadipate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.27 | - |
2-oxobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.27 | - |
hydroxyphenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.76 | - |
4-methyl-2-oxopentanoate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.78 | - |
2-oxovalerate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.08 | - |
indo-3-pyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.23 | - |
Mercaptopyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
1.75 | - |
2-oxo-4-methylthiobutyrate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
2.62 | - |
phenylpyruvate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.13 | - |
2-oxocaproate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
3.27 | - |
L-kynurenine | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
6.24 | - |
2-oxoglutarate | in 100 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens |