Cloned (Comment) | Organism |
---|---|
expression as GST-tagged enzyme in Escherichia coli strain BL21 | Human alphaherpesvirus 3 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant GST-tagged enzyme complexed with ADP and E-5-(2-bromovinyl)-2'-deoxythymidine, sitting drop vapour diffusion method, 21°C, mixing of 0.001 ml of enzyme and reservoir solution, the enzyme solution contains 8 mg/ml protein, 0.4 mM ADP, 0.2 mM E-5-(2-bromovinyl)-2'-deoxythymidine, and 0.6 mM MgCl2, reservoir solution contains 0.1 M sodium acetate, at either pH 4.5 or 5.0, and 6-11% w/v PEG 20000, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement method | Human alphaherpesvirus 3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | Human alphaherpesvirus 3 | drug activation by phosphorylation | ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + thymidine | Human alphaherpesvirus 3 | - |
ADP + thymidine 5'-phosphate | - |
? | |
additional information | Human alphaherpesvirus 3 | the viral enzyme shows a broader substrate specificity than the human host cell enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human alphaherpesvirus 3 | - |
i.e. human Herpes virus 3, VZV | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography | Human alphaherpesvirus 3 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | active site structure, substrate binding structures | Human alphaherpesvirus 3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | - |
Human alphaherpesvirus 3 | ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | drug activation by phosphorylation | Human alphaherpesvirus 3 | ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + thymidine | - |
Human alphaherpesvirus 3 | ADP + thymidine 5'-phosphate | - |
? | |
additional information | the viral enzyme shows a broader substrate specificity than the human host cell enzyme | Human alphaherpesvirus 3 | ? | - |
? | |
additional information | modeling of ganciclovir binding into the enzyme crystal structure, overview | Human alphaherpesvirus 3 | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Human alphaherpesvirus 3 |