Crystallization (Comment) | Organism |
---|---|
in complex with substrates, sitting drop vapor diffusion method, using M HEPES pH 7.5, 11% (v/v) hexane-1,6-diol, 25% (w/v) PEG400 | Trypanosoma brucei gambiense |
Protein Variants | Comment | Organism |
---|---|---|
D20A | about 95% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
E478A | about 25% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
M1A | about 90% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
R22A | about 60% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
R24A | about 30% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
R477A | inactive | Trypanosoma brucei gambiense |
T11V | inactive | Trypanosoma brucei gambiense |
T12V | less than 5% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
T273V | about 80% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
T276V | inactive | Trypanosoma brucei gambiense |
Y3F | about 80% activity compared to the wild type enzyme | Trypanosoma brucei gambiense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | Trypanosoma brucei gambiense | - |
ATP + glycerol | - |
r | |
ATP + glycerol | Trypanosoma brucei gambiense | - |
ADP + sn-glycerol 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei gambiense | D3KVM3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei gambiense | ATP + glycerol | - |
r | |
ATP + glycerol | - |
Trypanosoma brucei gambiense | ADP + sn-glycerol 3-phosphate | - |
r |