Protein Variants | Comment | Organism |
---|---|---|
additional information | co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode, function as improved amperometric triglyceride biosensor. An improved amperometric triglyceride (TG) biosensor is fabricated using lipaseNPs/GKNPs/GPONPs/PG electrode as the working electrode, Ag/AgCl as the standard electrode and Pt wire as auxiliary electrode. The biosensor shows optimum response within 2.5 s at pH 7.0 and temperature of 35°C. The biosensor measures current due to electrons generated at 0.1 V against Ag/AgCl, from H2O2, which is produced from triolein by co-immobilized enzyme nanoparticles (ENPs). The biosensor is evaluated and employed for determination of triglyceride in the serum of apparently healthy subject and persons suffering from hypertriglyceridemia. The biosensor loses 20% of its initial activity after continued uses over a period of 240 days, while being stored at 4°C. Construction of the biosensor, evaluation, and optimization, overview | Cellulomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cellulomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128000 | - |
gel filtration | Cellulomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | Cellulomonas sp. | - |
ADP + sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Cellulomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | 75 | purified enzyme, pH 9.8, 25°C | Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | - |
Cellulomonas sp. | ADP + sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
enzyme in biosensor on graphite electrode | Cellulomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme in biosensor on graphite electrode | Cellulomonas sp. |