Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
expression in Escherichia coli as N-terminally His-tagged fusion | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NADH | Saccharomyces cerevisiae | Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants | ADP + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | Q06892 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
cobalt chelate affinity column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
with GTP as phosphate donor | Saccharomyces cerevisiae |
0.5 | - |
with CTP as phosphate donor | Saccharomyces cerevisiae |
1.2 | - |
with ATP as phosphate donor | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Saccharomyces cerevisiae | ADP + NADP+ | - |
? | |
ATP + NADH | - |
Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
ATP + NADH | phosphorylates NADH with 2% of the specific activity of its NAD kinase activity | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
ATP + NADH | possesses 33% activity with NADH and ATP compared to the activity obtained with NAD and ATP | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
ATP + NADH | Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants | Saccharomyces cerevisiae | ADP + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADH kinase POS5, mitochondrial | - |
Saccharomyces cerevisiae |
Pos5 | - |
Saccharomyces cerevisiae |
Utr1 | - |
Saccharomyces cerevisiae |
YEF1 | - |
Saccharomyces cerevisiae |
YEL041W | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |