Literature summary for 2.7.1.90 extracted from

Frese, M.; Schatschneider, S.; Voss, J.; Vorhoelter, F.J.; Niehaus, K.
Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris (2014), Arch. Biochem. Biophys., 546, 53-63.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	neither inhibitory nor activating: oxaloacetic acid, glucose 6-phosphate, citric acid, isocitric acid, 2,3-bisphosphoglyceric acid and malic acid at 5 mM	Xanthomonas campestris pv. campestris

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Xanthomonas campestris pv. campestris

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	neither inhibitory nor activating: oxaloacetic acid, glucose 6-phosphate, citric acid, isocitric acid, 2,3-bisphosphoglyceric acid and malic acid at 5 mM	Xanthomonas campestris pv. campestris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	D-fructose 1,6-bisphosphate	pH 6.8, 25°C	Xanthomonas campestris pv. campestris
0.041	-	diphosphate	pH 6.8, 25°C	Xanthomonas campestris pv. campestris
0.2	-	D-fructose 6-phosphate	pH 6.8, 25°C	Xanthomonas campestris pv. campestris
2.5	-	phosphate	pH 6.8, 25°C	Xanthomonas campestris pv. campestris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44700	-	-	Xanthomonas campestris pv. campestris
45000	-	-	Xanthomonas campestris pv. campestris

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris pv. campestris	B0RP51	-	-
Xanthomonas campestris pv. campestris B100	B0RP51	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
58	-	synthesis of D-fructose 1,6-bisphosphate, pH 6.8, 25°C	Xanthomonas campestris pv. campestris
59	-	synthesis of disphosphate, pH 6.8, 25°C	Xanthomonas campestris pv. campestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	-	Xanthomonas campestris pv. campestris	phosphate + D-fructose 1,6-bisphosphate	1.08 molecules fructose 1,6-bisphosphate formed per consumed molecule of diphosphate	r
diphosphate + D-fructose 6-phosphate	-	Xanthomonas campestris pv. campestris B100	phosphate + D-fructose 1,6-bisphosphate	1.08 molecules fructose 1,6-bisphosphate formed per consumed molecule of diphosphate	r
additional information	no substrate: ATP	Xanthomonas campestris pv. campestris	?	-	?
additional information	no substrate: ATP	Xanthomonas campestris pv. campestris B100	?	-	?
phosphate + D-fructose 1,6-bisphosphate	-	Xanthomonas campestris pv. campestris	diphosphate + D-fructose 6-phosphate	-	r
phosphate + D-fructose 1,6-bisphosphate	-	Xanthomonas campestris pv. campestris B100	diphosphate + D-fructose 6-phosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGe, x * 44700, calculated	Xanthomonas campestris pv. campestris

Synonyms

Synonyms	Comment	Organism
PFKA	-	Xanthomonas campestris pv. campestris

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Xanthomonas campestris pv. campestris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	-	Xanthomonas campestris pv. campestris

General Information

General Information	Comment	Organism
physiological function	an enzyme deletion mutant shows 1.9% of the wild-type activity	Xanthomonas campestris pv. campestris

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Release 2023.1 (January 2023)