Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMpYSSV | CBP-based phosphopeptide, activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant GST-fusion Csk proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K361G/K362A | the mutant shows reduced phosphorylation activity compared to the wild-type enzyme, especially with substrate poly(Glu4-Tyr) | Homo sapiens |
additional information | construction of mutants DSH3 and DSH2 lacking the SH3 and SH2 domains, respectively, the mutants show decreased binding to phosphorylation sites of substrates compared to the wild-type enzyme, mutant DSH2 also shows reduced phosphorylation activity | Homo sapiens |
R389A | the mutant shows reduced phosphorylation activity compared to the wild-type enzyme, especially with substrate [kdSrc kinase]-L-tyrosine | Homo sapiens |
W188A | the mutant shows increased binding to phosphorylation sites of substrates but reduced phosphorylation activity compared to the wild-type enzyme, especially with substrate poly(Glu4-Tyr) | Homo sapiens |
W188F | the mutant shows increased binding to phosphorylation sites of substrates but reduced phosphorylation activity compared to the wild-type enzyme, especially with substrate poly(Glu4-Tyr) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant wild-type Csk | Homo sapiens | |
0.017 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant W188F Csk mutant | Homo sapiens | |
0.027 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant DSH3 Csk mutant | Homo sapiens | |
0.032 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant K361G/K362A Csk mutant | Homo sapiens | |
0.042 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant DSH2 Csk mutant | Homo sapiens | |
0.068 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant W188A Csk mutant | Homo sapiens | |
0.15 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant wild-type Csk | Homo sapiens | |
0.164 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant DSH2 Csk mutant | Homo sapiens | |
0.204 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant W188A Csk mutant | Homo sapiens | |
0.299 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant W188F Csk mutant | Homo sapiens | |
0.328 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant DSH3 Csk mutant | Homo sapiens | |
0.659 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant K361G/K362A Csk mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GST-fusion Csk proteins by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
ATP + [kdSrc kinase]-L-tyrosine | i.e. kinase-defective chicken Src mutant K295M | Homo sapiens | ADP + [kdSrc kinase]-L-tyrosine phosphate | - |
? | |
additional information | Csk-substrate interaction requires the SH2 and SH3 enzyme domains | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Csk structure analysis, PDB code 1K9A, compared to tyrosine kinase Hsk, PDB code 1QCF | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Csk | - |
Homo sapiens |
Protein tyrosine kinase | - |
Homo sapiens |
PTK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding lobe structure | Homo sapiens |