Any feedback?
Literature summary for 2.7.10.2 extracted from
- Expression, purification, and biochemical characterization of Chk, a soluble protein tyrosine kinase (2003), Protein Expr. Purif., 29, 148-155.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of Chk as soluble dual affinity tag fusion protein, with a GST-tag at the N-terminus and a Strep-tag at the C-terminus, in Escherichia coli strain DH5alpha | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d] pyrimidine | i.e. pp2, inhibition of Csk involves Thr265, no inhibition of Chk | Homo sapiens |  |
| Mn2+ | inhibitory above 1 mM | Homo sapiens | |
| additional information | enzyme is cleaved by several proteases such as thrombin | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
poly(Glu4-Tyr) | recombinant GST-fusion Chk, 12 mM Mg2+, no Mn2+ | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Chk requires about 10 mM Mg2+ for full activity | Homo sapiens | |
| Mn2+ | optimal at 0.5 mM, inhibitory above 1 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
| 77000 | - |
x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Strep-tagged GST-fusion Chk from Escherichia coli strain DH5alpha 6fold by glutathione and streptavidin affinity chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0173 | - |
purified recombinant Strep-tagged GST-fusion Chk | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + lysozyme-L-tyrosine | substrate of Src, low activity with Csk or Chk | Homo sapiens | ADP + lysozyme-L-tyrosine phosphate | - |
? | |
| ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
| ATP + [kdSrc kinase]-L-tyrosine | i.e. kinase-defective Src, substrate of Chk and Csk, inactivation mechanism of Src, overview | Homo sapiens | ADP + [kdSrc kinase]-L-tyrosine phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHK | - |
Homo sapiens |
| Csk | - |
Homo sapiens |
| More | Chk belongs to the Src family of PTKs | Homo sapiens |
| Protein tyrosine kinase | - |
Homo sapiens |
| PTK | - |
Homo sapiens |
| Src | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
