Cloned (Comment) | Organism |
---|---|
gene kpnK, quantitative real-time RT-PCR expression analysis of wild-type and mutant enzymes, recombinant expression in Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the kpnK deletion mutant in strain MGH78578 | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | the enzyme is a soluble protein with no transmembrane domains | Klebsiella pneumoniae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | low activity, can only partly substitute for Mn2+ | Klebsiella pneumoniae | |
Mn2+ | enzyme KpnK is dependent on Mn2+ | Klebsiella pneumoniae | |
additional information | no activity with Zn2+, Ni2+, and Mg2+ | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity | ? | - |
? | |
additional information | Klebsiella pneumoniae MGH78578 | KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
gene kpnK or KPN_04169 | - |
Klebsiella pneumoniae MGH78578 | - |
gene kpnK or KPN_04169 | - |
Purification (Comment) | Organism |
---|---|
recombinant KpnK from Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + chicken basic protein | - |
Klebsiella pneumoniae | ADP + phosphorylated chicken basic protein | - |
? | |
ATP + chicken basic protein | - |
Klebsiella pneumoniae MGH78578 | ADP + phosphorylated chicken basic protein | - |
? | |
additional information | KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity | Klebsiella pneumoniae | ? | - |
? | |
additional information | KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity | Klebsiella pneumoniae MGH78578 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ESTK | - |
Klebsiella pneumoniae |
eukaryotic-like serine/threonine kinase | - |
Klebsiella pneumoniae |
KpnK | - |
Klebsiella pneumoniae |
Ser/Thr kinase | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | activity range | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | narrow activity range | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Klebsiella pneumoniae |
Organism | Comment | Expression |
---|---|---|
Klebsiella pneumoniae | gene knpK is induced by oxidative and nitrosative stress and other stress-associated challenge, overview | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the DkpnK mutant exhibits higher sensitivity to gastrointestinal and oxidative stresses. Deletion of kpnK result in a two to threefold increased susceptibility towards imipenem, cefepime, ceftriaxone and ceftazidime, gene kpnK participates in antibiotic resistance. Deletion of gene kpnK influences active efflux in Klebsiella pneumoniae. The downregulation of the MDR efflux pumps might be the molecular mechanism underlying the sensitivity of kpnK mutant to hydrophobic agents | Klebsiella pneumoniae |
physiological function | involvement of ESTK in regulating bacterial physiology, stress response and drug resistance. Roles of gene kpnK in oxidative and nitrosative stress tolerance and in other stress-associated challenge, overview. Additional regulatory role of KpnK on MDR efflux pumps | Klebsiella pneumoniae |