Cloned (Comment) | Organism |
---|---|
expression of functional residues 1-25 of the enzyme as His-tagged peptide RK25 in Escherichia coli strain BL21(DE3) | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
recoverin | calcium-induced inhibition, structural mechanism, recoverin serves as a calcium sensor that regulates rhodopsin kinase activity, binding structures, overview, NMR structure determination and analysis of the ternary complex RK25-Ca2+-recoverin | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | affects enzyme binding to rhodopsin, induces binding of recoverin to the enzyme inhibiting its activity, NMR structure determination and analysis of the ternary complex RK25-Ca2+-recoverin | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme fragment RK25 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
retina | - |
Bos taurus | - |
retinal rod | - |
Bos taurus | - |
Subunits | Comment | Organism |
---|---|---|
More | NMR structure determination and analysis of the ternary complex RK25-Ca2+-recoverin | Bos taurus |