Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
the phosphorylated cytoplasmic domain of DesK is crystallized by hanging drop vapor diffusion, using 10% (w/v) PEG 3000, 0.1 M CHES, pH 9.5, and 10 mM MgCl2, at 18°C | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
H188V | the mutant retains the phosphatase activity of the wild type protein, the mutation triggers formation of an N-terminal 2-helical coiled-coil and extensive dimerization and histidine phosphotransfer domain-ATP-binding domain association | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein L-histidine | Bacillus subtilis | - |
ADP + protein N-phospho-L-histidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34757 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and Superdex 75 gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein L-histidine | - |
Bacillus subtilis | ADP + protein N-phospho-L-histidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
DesK | - |
Bacillus subtilis |
thermosensor histidine kinase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | DesK is cold-activated | up |