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Literature summary for 2.7.13.3 extracted from

  • Horstmann, N.; Sahasrabhojane, P.; Saldana, M.; Ajami, N.J.; Flores, A.R.; Sumby, P.; Liu, C.G.; Yao, H.; Su, X.; Thompson, E.; Shelburne, S.A.
    Characterization of the effect of the histidine kinase CovS on response regulator phosphorylation in group A Streptococcus (2015), Infect. Immun., 83, 1068-1077.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment) Organism
gene covS, dNA and amino acid sequence determination and analysis Streptococcus pyogenes MGAS10870

Protein Variants

Protein Variants Comment Organism
E281A site-directed mutagenesis Streptococcus pyogenes MGAS10870
G457V site-directed mutagenesis Streptococcus pyogenes MGAS10870
H280V site-directed mutagenesis Streptococcus pyogenes MGAS10870
I332V site-directed mutagenesis Streptococcus pyogenes MGAS10870
additional information genetic inactivation of covS decreases but does not eliminate CovR phosphorylation Streptococcus pyogenes MGAS10870
T284A site-directed mutagenesis Streptococcus pyogenes MGAS10870

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pyogenes MGAS2221
Mg2+ required, activates CovR phosphorylation at high concentration. High-Mg2+ conditions increase phosphorylated CovR levels dependent on the presence of an intact CovS protein without influencing CovR production Streptococcus pyogenes MGAS10870

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + CovR L-histidine Streptococcus pyogenes MGAS10870
-
 ADP + CovR N-phospho-L-histidine
-
 ?
ATP + CovR L-histidine Streptococcus pyogenes MGAS2221
-
 ADP + CovR N-phospho-L-histidine
-
 ?
ATP + protein L-histidine Streptococcus pyogenes MGAS10870
-
 ADP + protein N-phospho-L-histidine
-
 ?
ATP + protein L-histidine Streptococcus pyogenes MGAS2221
-
 ADP + protein N-phospho-L-histidine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes MGAS10870
-
 serotype M3
-
Streptococcus pyogenes MGAS2221
-
 serotype M1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CovR L-histidine
-
 Streptococcus pyogenes MGAS10870 ADP + CovR N-phospho-L-histidine
-
 ?
ATP + CovR L-histidine
-
 Streptococcus pyogenes MGAS2221 ADP + CovR N-phospho-L-histidine
-
 ?
ATP + protein L-histidine
-
 Streptococcus pyogenes MGAS10870 ADP + protein N-phospho-L-histidine
-
 ?
ATP + protein L-histidine
-
 Streptococcus pyogenes MGAS2221 ADP + protein N-phospho-L-histidine
-
 ?

Synonyms

Synonyms Comment Organism
CovS
-
 Streptococcus pyogenes MGAS10870
CovS
-
 Streptococcus pyogenes MGAS2221

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Streptococcus pyogenes MGAS10870
ATP
-
 Streptococcus pyogenes MGAS2221

General Information

General Information Comment Organism
malfunction a group A streptococci strain selectively deficient in CovS phosphatase activity has a distinct transcriptome relative to that of its parental strain. Inactivation of CovS in the serotype M1 background results in a greater decrease in phosphorylated CovR levels and a greater increase in the transcript levels of CovR-repressed genes than does CovS inactivation in a serotype M3 strain Streptococcus pyogenes MGAS10870
malfunction a group A streptococci strain selectively deficient in CovS phosphatase activity has a distinct transcriptome relative to that of its parental strain. Inactivation of CovS in the serotype M1 background results in a greater decrease in phosphorylated CovR levels and a greater increase in the transcript levels of CovR-repressed genes than does CovS inactivation in a serotype M3 strain Streptococcus pyogenes MGAS2221
metabolism compared to a serotype M3 strain, serotype M1 GAS strains have high levels of phosphorylated CovR, low transcript levels of CovR-repressed genes, and strikingly different responses to environmental cues Streptococcus pyogenes MGAS2221
metabolism compared to a serotype M3 strain, serotype M1 GAS strains have high levels of phosphorylated CovR, low transcript levels of CovR-repressed genes, and strikingly different responses to environmental cues. Genetic inactivation of covS decreases but does not eliminate CovR phosphorylation Streptococcus pyogenes MGAS10870
physiological function two-component gene regulatory systems (TCSs) are a major mechanism by which bacteria respond to environmental stimuli and are critical to infectivity. The control of virulence regulator/sensor kinase (CovRS) TCS is central to the virulence of the major human pathogen group A Streptococcus (GAS). In the system, the histidine kinase CovS primarily serves to phosphorylate CovR, thereby resulting in the repression of virulence factor-encoding genes. Both CovS kinase and phosphatase activities influence the CovR phosphorylation status. Serotype M1 GAS strains have high rates of spontaneous mutations in covS during invasive GAS infection, thus providing a link between TCS molecular function and the epidemiology of deadly bacterial infections Streptococcus pyogenes MGAS10870
physiological function two-component gene regulatory systems (TCSs) are a major mechanism by which bacteria respond to environmental stimuli and are critical to infectivity. The control of virulence regulator/sensor kinase (CovRS) TCS is central to the virulence of the major human pathogen group A Streptococcus (GAS). In the system, the histidine kinase CovS primarily serves to phosphorylate CovR, thereby resulting in the repression of virulence factor-encoding genes. Both CovS kinase and phosphatase activities influence the CovR phosphorylation status. Serotype M1 GAS strains have high rates of spontaneous mutations in covS during invasive GAS infection, thus providing a link between TCS molecular function and the epidemiology of deadly bacterial infections Streptococcus pyogenes MGAS2221