Cloned (Comment) | Organism |
---|---|
full length and truncated enzyme lacking the first 22 amino acids expressed in Escherichia coli BL21 C41 (DE3) | Equid alphaherpesvirus 4 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Equid alphaherpesvirus 4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis | Equid alphaherpesvirus 4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | Herpes simplex virus | - |
ADP + TMP | - |
? | |
ATP + thymidine | Equid alphaherpesvirus 4 | - |
ADP + TMP | - |
? | |
ATP + TMP | Herpes simplex virus | - |
ADP + TDP | - |
? | |
ATP + TMP | Equid alphaherpesvirus 4 | - |
ADP + TDP | - |
? | |
additional information | Herpes simplex virus | multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues | ? | - |
? | |
additional information | Equid alphaherpesvirus 4 | multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equid alphaherpesvirus 4 | - |
virus type 4 | - |
Herpes simplex virus | - |
virus type 1 | - |
Purification (Comment) | Organism |
---|---|
truncated recombinant enzyme, full length enzyme could not be crystallized | Equid alphaherpesvirus 4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | - |
Herpes simplex virus | ADP + TMP | - |
? | |
ATP + thymidine | - |
Equid alphaherpesvirus 4 | ADP + TMP | - |
? | |
ATP + TMP | - |
Herpes simplex virus | ADP + TDP | - |
? | |
ATP + TMP | - |
Equid alphaherpesvirus 4 | ADP + TDP | - |
? | |
additional information | multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues | Herpes simplex virus | ? | - |
? | |
additional information | multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues | Equid alphaherpesvirus 4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis | Equid alphaherpesvirus 4 |