Application | Comment | Organism |
---|---|---|
drug development | the enzyme might be a target for inhibitor design in galactosemia treatment | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structure determination and analysis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
ADP-glucose | - |
Homo sapiens | |
alpha-D-galactose 1-phosphate | competitive substrate inhibition in the forward reaction | Escherichia coli | |
alpha-D-galactose 1-phosphate | competitive substrate inhibition in the forward reaction | Homo sapiens | |
ATP | - |
Homo sapiens | |
CDP-glucose | - |
Homo sapiens | |
diethyl dicarbonate | inactivates the enzyme, reversable by hydroxylamine | Escherichia coli | |
GDP-glucose | - |
Homo sapiens | |
TDP-glucose | - |
Homo sapiens | |
UDP | - |
Homo sapiens | |
UDP-galactose | the product of the forward reaction is a competitive inhibitor with respect to UDP-glucose and a mixed inhibitor with respect to galactose 1-phosphate | Escherichia coli | |
UDP-galactose | the product of the forward reaction is a competitive inhibitor with respect to UDP-glucose and a mixed inhibitor with respect to galactose 1-phosphate | Homo sapiens | |
UDP-glucuronic acid | - |
Homo sapiens | |
UDP-mannose | - |
Homo sapiens | |
UDP-xylose | - |
Homo sapiens | |
UMP | - |
Homo sapiens | |
Uracil | - |
Homo sapiens | |
uridine | - |
Homo sapiens | |
UTP | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme homodimer contains one zinc (II) and one iron (II) ion per subunit, pyramidial iron-binding site, structure, overview | Escherichia coli | |
Zn2+ | the enzyme homodimer contains one zinc (II) and one iron (II) ion per subunit, tetrahedral zinc binding site, structure, overview | Escherichia coli | |
Zn2+ | the zinc co-orientating residues are not conserved and therefore, it may only bind one metal ion per monomer | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | Escherichia coli | - |
alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | Homo sapiens | - |
alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | ping-pong catalytic mechanism, a UMP group is transferred from UDP-glucose to His186 in the active site of the enzyme, overview | Homo sapiens | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | ping-pong catalytic mechanism, overview. A UMP group is transferred from UDP-glucose to His166 in the active site of the enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has a strict requirement for UDP-glucose or UDP-galactose as substrates, while GDP-glucose, ADP-glucose, TDP-glucose, CDP-glucose, UDPxylose, and UDP-mannose are all unable to support activity | Escherichia coli | ? | - |
? | |
additional information | the enzyme has a strict requirement for UDP-glucose or UDP-galactose as substrates, while GDP-glucose, ADP-glucose, TDP-glucose, CDP-glucose, UDPxylose, and UDP-mannose are all unable to support activity | Homo sapiens | ? | - |
? | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
galactose 1-phosphate uridylyltransferase | - |
Escherichia coli |
galactose 1-phosphate uridylyltransferase | - |
Homo sapiens |
GALT | - |
Escherichia coli |
GALT | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | GALT belongs to the histidine triad family of transferases | Escherichia coli |
evolution | GALT belongs to the histidine triad family of transferases | Homo sapiens |
malfunction | reduced galactose 1-phosphate uridylyltransferase activity is associated with the genetic disease type I galactosemia. Enzyme-deficiency results in an increase in the cellular concentration of galactose 1-phosphate. The accumulation of this toxic metabolite, combined with aberrant glycoprotein and glycolipid biosynthesis, is likely to be the major factor in molecular pathology | Homo sapiens |
metabolism | the enzyme catalyzes a critical step in the Leloir pathway, overview | Escherichia coli |
metabolism | the enzyme catalyzes a critical step in the Leloir pathway, overview | Homo sapiens |