Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight stimulation | Neurospora crassa | |
dithioerythritol | highly stimulating | Neurospora crassa | |
dithiothreitol | highly stimulating | Neurospora crassa | |
glutathione | slight stimulation | Neurospora crassa |
General Stability | Organism |
---|---|
dithiothreitol stabilizes, activity lost during storage can be recovered by addition of dithiothreitol | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
UDP-N-acetyl-D-glucosamine | pH 7.5, 30°C | Neurospora crassa | |
5.4 | - |
diphosphate | pH 7.5, 30°C | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ | Neurospora crassa | |
Mg2+ | required for maximum activity | Neurospora crassa | |
Mn2+ | can partially replace Mg2+ | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa IFO 6178 | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa IFO 6178 | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Neurospora crassa IFO 6178 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.66 | - |
purified enzyme, pH 7.5, 30°C | Neurospora crassa |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer, pH 7.5, stable for at least 2 weeks | Neurospora crassa |
4°C, phosphate buffer, pH 7.5, 0.01 mM dithiothreitol, stable for at least 2 weeks | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | highly specific, no activity with any other sugar nucloetide tested | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | highly specific, no activity with any other sugar nucloetide tested | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, 50% of activity lost in the absence of dithiothreitol, 20% of activity lost in presence of dithiothreitol | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Neurospora crassa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | most stable | Neurospora crassa |