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Literature summary for 2.7.7.4 extracted from

  • Osslund, T.; Chandler, C.; Segel, I.H.
    ATP sulfurylase from higher plants. Purification and preliminary kinetics studies on the cabbage leaf enzyme (1982), Plant Physiol., 70, 39-45.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.4

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate
-
 Brassica capitata
ClO3-
-
 Brassica capitata
MgATP2-
-
 Brassica capitata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00071
-
 diphosphate pH 8.0, 30°C Brassica capitata
0.001
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Brassica capitata
0.31
-
 ATP pH 8.0, 30°C, SO42- as substrate Brassica capitata
0.31
-
 ATP ATP in form of MgATP2- Brassica capitata
0.33
-
 ATP pH 8.0, 30°C, MoO42- as substrate Brassica capitata
0.33
-
 ATP ATP in form of MgATP2- Brassica capitata
0.64
-
 MoO42- pH 8.0, 30°C Brassica capitata
0.87
-
 SO42- pH 8.0, 30°C Brassica capitata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
 glycerol density gradient centrifugation Brassica capitata

Organism

Organism UniProt Comment Textmining
Brassica capitata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica capitata

Reaction

Reaction Comment Organism Reaction ID
ATP + sulfate = diphosphate + adenylyl sulfate obligatory ordered kinetic mechanism with MgATP2- adding before MoO42- or SO42- and Mg-diphosphate leaving before AMP + MoO42- or adenosine 5'-phosphosulfate Brassica capitata
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica capitata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
 synthesis of adenosine 5'-phosphosulfate Brassica capitata
4.3
-
 Mg-diphosphate-MgATP2-exchange Brassica capitata
30
-
-
 Brassica capitata
38
-
 molybdolysis, and synthesis of MgATP2- Brassica capitata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + molybdate
-
 Brassica capitata diphosphate + adenylyl molybdate
-
 r
ATP + MoO42-
-
 Brassica capitata AMP + adenylylmolybdate
-
 ?
ATP + sulfate
-
 Brassica capitata diphosphate + adenylylsulfate
-
 r
ATP + sulfate
-
 Brassica capitata diphosphate + adenylyl sulfate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 56000-57000, 2 band of 56000 Da and of 57000 Da, the 56000 Da protein perhaps is a partially proteolyzed subunit, SDS-PAGE Brassica capitata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13
-
 ATP pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate Brassica capitata
3.13
-
 SO42- pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate Brassica capitata
36
-
 ATP pH 8.0, 30°C, molybdolysis Brassica capitata
36
-
 diphosphate pH 8.0, 30°C, synthesis of ATP Brassica capitata
36
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, synthesis of ATP Brassica capitata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Brassica capitata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0015
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C, product inhibitor Brassica capitata
0.3
-
 ClO3- pH 8.0, 30°C Brassica capitata
0.75 1.3 MgATP2- pH 8.0, 30°C Brassica capitata