Cloned (Comment) | Organism |
---|---|
expression of His-tagged subunits PA and PB1 in Escherichia coli | influenza A virus |
Crystallization (Comment) | Organism |
---|---|
a large fragment of subunit PA bound to a fragment of subunit PB1, hanging dropn vapour diffusion method, 20°C, mixing of 0.001 ml of protein solution, containing 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM sodium chloride, and 0.001 ml of reservoir solution consisting of 100 mM Tris-HCl, pH 7.5, and 2.4 M sodium formate, X-ray diffraction structure determination and analysis at 2.3 A resolution | influenza A virus |
Protein Variants | Comment | Organism |
---|---|---|
L640D | the mutant of the PA subunit shows reduced transcriptional activity compared to the wild-type enzyme | influenza A virus |
L666D | the mutant of the PA subunit shows reduced transcriptional activity compared to the wild-type enzyme | influenza A virus |
V636S | the mutant of the PA subunit shows reduced transcriptional activity compared to the wild-type enzyme | influenza A virus |
W706A | the mutant of the PA subunit shows reduced transcriptional activity compared to the wild-type enzyme | influenza A virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
influenza A virus | P03433 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged subunits PA and PB1 from Escherichia coli by nickel affinity chromatography | influenza A virus |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | the three subunits, PB1, PB2 and PA, are all required for both transcription and replication, PB1 carries the polymerase active site, PB2 includes the capped-RNA recognition domain, and PA, whose C-terminal domain consists of 13 alpha-helices and 9 beta-strands, is involved in assembly of the functional complex. The subunit interface is important for viral replication, overview | influenza A virus |
Synonyms | Comment | Organism |
---|---|---|
RNA polymerase | - |
influenza A virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | influenza A virus |