Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | activation at 5-15% v/v | Moloney murine leukemia virus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged MMLV RT in Escherichia coli strain BL21(DE3) | Moloney murine leukemia virus |
expression of N-terminally His6-tagged HIV-1 group M and O RT subunits p51 and p66 in Escherichia coli strain BL21(DE3) | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
K65R | a HIV-1 O RT variant that shows increased fidelity and stability compared to the wild-type enzyme | Human immunodeficiency virus 1 |
K65R/V75I | a HIV-1 O RT variant that shows increased fidelity and stability compared to the wild-type enzyme | Human immunodeficiency virus 1 |
R78A | a HIV-1 O RT variant that shows increased fidelity and stability compared to the wild-type enzyme | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Moloney murine leukemia virus | |
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Human immunodeficiency virus 1 | |
0.0013 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme, HIV-1 O RT | Human immunodeficiency virus 1 | |
0.0014 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme, HIV-1 M RT | Human immunodeficiency virus 1 | |
0.0056 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme | Moloney murine leukemia virus | |
0.02 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme, HIV-1 M RT | Human immunodeficiency virus 1 | |
0.03 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme, HIV-1 O RT | Human immunodeficiency virus 1 | |
0.25 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme | Moloney murine leukemia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Moloney murine leukemia virus | |
Mg2+ | required | Human immunodeficiency virus 1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 66000 + 1 * 51000 or 2 * 66000 or 2 * 51000 | Human immunodeficiency virus 1 |
66000 | - |
1 * 66000 + 1 * 51000 or 2 * 66000 or 2 * 51000 | Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Moloney murine leukemia virus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Human immunodeficiency virus 1 | - |
diphosphate + DNAn+1 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 50% loss of activity at 15% v/v, inactivation at 25% v/v | Human immunodeficiency virus 1 |
DMSO | stable up to 15% v/v, 50% activation at 5-15% v/v, inactivation at 25% v/v | Moloney murine leukemia virus |
formamide | 50% loss of activity at 17.5% v/v, inactivation at 15% v/v | Moloney murine leukemia virus |
formamide | HIV-1 RT has low susceptibility to formamide, stable up to 15% v/v, inactivation at 25% v/v | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
group O and M virus, Group O infection is restricted to Cameroon and neighboring countries in West Central Africa, while Group M infection is spread all over the world | - |
Moloney murine leukemia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MMLV RT from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, anion exchange and nickel affinity chromatography | Moloney murine leukemia virus |
recombinant N-terminally His6-tagged HIV-1 group M and O RT subunits p51 and p66 from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, anion exchange and nickel affinity chromatography | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | incorporation of dTTP into poly(rA)-p(dT)15 | Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | incorporation of dTTP into poly(rA)-p(dT)15 | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
additional information | HIV-1 RT has high substrate affinity and low susceptibility to formamide | Moloney murine leukemia virus | ? | - |
? | |
additional information | HIV-1 RT has high substrate affinity and low susceptibility to formamide, and low fidelity. Extremely high fidelity is not required for detection of a target RNA in RT-polymerase chain reaction and RNA-specific amplification, and an isothermal reaction that is widely used in clinical diagnosis, in which RT synthesizes promoter-bearing double-stranded DNA with the help of its RNase H activity | Human immunodeficiency virus 1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Moloney murine leukemia virus |
dimer | 1 * 66000 + 1 * 51000 or 2 * 66000 or 2 * 51000 | Human immunodeficiency virus 1 |
More | HIV-1 RT exists as a heterodimer as well as a homodimer | Human immunodeficiency virus 1 |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 M RT | - |
Human immunodeficiency virus 1 |
HIV-1 O RT | - |
Human immunodeficiency virus 1 |
human immunodeficiency virus type 1 reverse transcriptase | - |
Human immunodeficiency virus 1 |
MMLV RT | - |
Moloney murine leukemia virus |
Moloney murine leukemia virus RT | - |
Moloney murine leukemia virus |
reverse transcriptase | - |
Moloney murine leukemia virus |
reverse transcriptase | - |
Human immunodeficiency virus 1 |
RT | - |
Moloney murine leukemia virus |
RT | - |
Human immunodeficiency virus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | assay at | Moloney murine leukemia virus |
25 | 37 | assay at | Human immunodeficiency virus 1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme, HIV-1 M RT | Human immunodeficiency virus 1 | |
0.35 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme, HIV-1 M RT | Human immunodeficiency virus 1 | |
1.1 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme, HIV-1 O RT | Human immunodeficiency virus 1 | |
1.1 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme, HIV-1 O RT | Human immunodeficiency virus 1 | |
29 | - |
dTTP | pH 8.3, 25°C, recombinant enzyme | Moloney murine leukemia virus | |
29 | - |
DNAn | pH 8.3, 25°C, recombinant enzyme | Moloney murine leukemia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Moloney murine leukemia virus |
8.3 | - |
assay at | Human immunodeficiency virus 1 |