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Literature summary for 2.7.7.49 extracted from

  • De Wit, F.; Pillalamarri, S.R.; Sebastian-Martin, A.; Venkatesham, A.; Van Aerschot, A.; Debyser, Z.
    Design of reverse transcriptase-specific nucleosides to visualize early steps of HIV-1 replication by click labeling (2019), J. Biol. Chem., 294, 11863-11875 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.49

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00082
-
 dATP pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.00133
-
 dATP pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.0016
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.00202
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.00317
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.00324
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.00389
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.00822
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.01365
-
 ((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
dATP + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
reverse transcriptase
-
 Human immunodeficiency virus 1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0195
-
 ((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.028
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.0295
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.03
-
 dATP pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.032
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
0.042
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.047
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.0545
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
0.067
-
 dATP pH 8.0, 37°C, RNA template Human immunodeficiency virus 1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.6
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
7.2
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
9.3
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
14.3
-
 ((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
14.5
-
 ((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
20
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
20.7
-
 ((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate pH 8.0, 37°C, RNA template Human immunodeficiency virus 1
36.6
-
 dATP pH 8.0, 37°C, DNA template Human immunodeficiency virus 1
50.38
-
 dATP pH 8.0, 37°C, RNA template Human immunodeficiency virus 1