Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus | |
thermine | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | in presence of 10 mM MgCl2 | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus | |
Mg2+ | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
92000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
140000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
170000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
HB8 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
HB8 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | calf thymus DNA as template | Thermus thermophilus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Thermus thermophilus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | calf thymus DNA as template | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermus thermophilus |