Crystallization (Comment) | Organism |
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hanging drop method, crystals of the enzyme in complexes with DNA (the binary complex) in the presence or absence of an incoming nucleotide are analyzed by Raman microscopy. 13C- and 15N-labeled d*CTP, or unlabeled dCTP, are soaked into the binary crystals with G as the templating base. In the presence of the catalytic metal ions, Mg2+ and Mn2+, nucleotide incorporation is detected by the disappearance of the triphosphate band of dCTP and the retention of *C modes in the crystal following soaking out of noncovalently bound C(or *C)TP. The addition of the second coded base, thymine, is observed by adding cognate dTTP to the crystal following a single d*CTP addition. Adding these two bases caused visible damage to the crystal that is possibly caused by protein and/or DNA conformational change within the crystal. When d*CTP is soaked into the Dpo4 crystal in the absence of Mn2+ or Mg2+, the primer extension reaction does not occur. Instead, a ternary protein/template/d*CTP complex is formed | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Synonyms | Comment | Organism |
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Dpo4 | - |
Saccharolobus solfataricus |