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Literature summary for 2.7.7.97 extracted from
- Combined purification of actinomycin synthetase I and 3-hydroxyanthranilic acid 4-methyltransferase from Streptomyces antibioticus (1993), J. Biol. Chem., 268, 6831-6834.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Streptomyces antibioticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxy-4-methylanthranilate | Streptomyces antibioticus | - |
diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, phenyl Sepharose column chromatography, QAE-Sepharose column chromatography, and Sephacryl gel filtration | Streptomyces antibioticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces antibioticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0008 | - |
crude enzyme, at pH 7.5 and 37°C | Streptomyces antibioticus |
| 0.795 | - |
after 958fold purification, at pH 7.5 and 37°C | Streptomyces antibioticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxy-4-methylanthranilate | - |
Streptomyces antibioticus | diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
| ATP + 3-hydroxyanthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
| ATP + 3-hydroxybenzoate | - |
Streptomyces antibioticus | ? | - |
? | |
| ATP + anthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
| ATP + benzoate | - |
Streptomyces antibioticus | ? | - |
? | |
| ATP + glycine | low activity | Streptomyces antibioticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Streptomyces antibioticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACMSI | - |
Streptomyces antibioticus |
| actinomycin synthetase I | - |
Streptomyces antibioticus |
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