Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | 0.01-0.025 mM, stimulates activity of tunicamycin-resistant cells about 20-30% | Glycine max | |
phosphatidylglycerol | activity is slightly enhanced | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-mannose | slight | Glycine max | |
GTP | 0.4 mM, 25-30% inhibition, enzyme from tunicamycin-resistant cells, no inhibition of wild-type enzyme up to 0.4 mM | Glycine max | |
PCMB | the enzyme from tunicamycin-resistant cells is equally sensitive to tunicamycin as the wilde-type enzyme | Glycine max | |
UDP-GlcNAc | strong inhibition | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | the tunicamycin resistant cells have a greater than 40fold increase in the activity of the enzyme UDP-GlcNAc:dolichyl-P GlcNAc1P transferase. Increase in enzyme activity is due to an increased production of the enzyme | Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Glycine max | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme | Glycine max |
40 | - |
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme | Glycine max |