BRENDA - Enzyme Database
show all sequences of 2.7.8.26

Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: evidence that dephosphorylation of adenosylcobalamin-5-phosphate by the CobC phosphatase is the last step of the pathway

Zayas, C.L.; Escalante-Semerena, J.C.; J. Bacteriol. 189, 2210-2218 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli strain C41(DE3)
Salmonella enterica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
Salmonella enterica
-
GMP + adenosylcobalamin + phosphate + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica
-
serovar typhimurium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
adenosylcobinamide-GDP + alpha-ribazole
-
674322
Salmonella enterica
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
alpha-ribazole-5-phosphate is a more preferred substrate compared to alpha-ribazole
674322
Salmonella enterica
GMP + adenosylcobalamin 5'-phosphate
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
-
674322
Salmonella enterica
GMP + adenosylcobalamin + phosphate + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
adenosylcobalamin-5-phosphate synthase
-
Salmonella enterica
AdoCbl-5-P synthase
-
Salmonella enterica
CobS
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain C41(DE3)
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
Salmonella enterica
-
GMP + adenosylcobalamin + phosphate + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
-
674322
Salmonella enterica
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
alpha-ribazole-5-phosphate is a more preferred substrate compared to alpha-ribazole
674322
Salmonella enterica
GMP + adenosylcobalamin 5'-phosphate
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
-
674322
Salmonella enterica
GMP + adenosylcobalamin + phosphate + H+
-
-
-
?
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674322
Zayas
Reassessment of the late steps ...
Salmonella enterica
J. Bacteriol.
189
2210-2218
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
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3
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674341
Zayas
The CbiB protein of Salmonella ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
189
7697-7708
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
8
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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2
-
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1
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
662778
Maggio-Hall
The last step in coenzyme B(12 ...
Methanothermobacter thermautotrophicus, Salmonella enterica
Microbiology
150
1385-1395
2004
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-
1
-
-
-
-
-
2
-
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-
-
10
-
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1
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
644450
Maggio-Hall
In vitro synthesis of the nucl ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
96
11798-11803
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
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1
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3
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2
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1
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2
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1
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1
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3
-
-
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-
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-
-
-
-
-
-
-
-
-
656416
Abd-Rahman
-
Gene cloning and sequence anal ...
Pyrococcus sp., Pyrococcus sp. KOD1
J. Ferment. Bioeng.
83
109-112
1997
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-
1
-
-
-
-
-
-
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1
2
-
6
-
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2
1
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1
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1
2
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2
1
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-
-
-
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-
489779
Cameron
Genetic analysis, nucleotide s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6066-6073
1991
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1
-
-
-
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3
-
-
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2
-
1
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1
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2
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1
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1
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3
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2
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1
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