General Stability | Organism |
---|---|
stable to freezing, aggregates on thawing | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Gallus gallus | |
CDP | strong inhibition | Gallus gallus | |
CMP | competitive inhibition to CDPethanolamine, non-competitive inhibition to L-serine | Gallus gallus | |
iodoacetamide | 1 mM, 6% remaining activity | Gallus gallus | |
iodoacetate | 1 mM, 42% remaining activity | Gallus gallus | |
L-alanine | 27-34% inhibition of the reaction | Gallus gallus | |
L-homoserine | 27-34% inhibition of the reaction | Gallus gallus | |
L-threonine | 27-34% inhibition of the reaction | Gallus gallus | |
Methylmercuric bromide | 0.1 mM, 6% remaining activity | Gallus gallus | |
Methylmercuric iodide | 0.1 mM, 3% remaining activity | Gallus gallus | |
additional information | iodoacetate is less effective as inhibitor, DFP is no inhibitor | Gallus gallus | |
N-Acetylimidazole | 10 mM, 8% remaining activity | Gallus gallus | |
N-ethylmaleimide | 0.2 mM, 25% remaining activity | Gallus gallus | |
p-hydroxymercuribenzoate | 0.1 mM, 2% remaining activity | Gallus gallus | |
phenylmercuric acetate | 0.1 mM, 2% remaining activity | Gallus gallus | |
Sulfanilic acid | diazotized, 0.2 mM, 36% remaining activity | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
CMP-aminoethylphosphonate | pH 7.0, 37°C | Gallus gallus | |
0.085 | - |
CDP-ethanolamine | pH 7.0, 37°C | Gallus gallus | |
1 | - |
L-serine | pH 7.0, 37°C | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Gallus gallus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Gallus gallus | |
Mg2+ | bivalent cations are essential for activity, Mg2+ is most effective | Gallus gallus | |
Mn2+ | - |
Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-ethanolamine + L-serine | Gallus gallus | - |
CMP + L-serine-ethanolamine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Purification (Comment) | Organism |
---|---|
partial | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Gallus gallus | - |
intestinal mucosa | - |
Gallus gallus | - |
kidney | - |
Gallus gallus | - |
additional information | no activity in liver, spleen, brain, oviduct, blood, and heart | Gallus gallus | - |
pancreas | - |
Gallus gallus | - |
Storage Stability | Organism |
---|---|
0°C, stable to freezing | Gallus gallus |
4°C, enzyme preparartion remains active | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-2-amino-2-methylpropanol + L-serine | - |
Gallus gallus | ? | - |
? | |
CDP-ethanolamine + DL-alpha-methylserine | - |
Gallus gallus | ? | - |
? | |
CDP-ethanolamine + L-serine | - |
Gallus gallus | CMP + L-serine-ethanolamine phosphate | - |
? | |
CDP-ethanolamine + L-serine | absolutely specific for serine, both stereoisomers are active | Gallus gallus | CMP + L-serine-ethanolamine phosphate | - |
? | |
CMP-aminoethylphosphonate + L-serine | - |
Gallus gallus | ? | - |
? | |
additional information | CDP-choline and CDP-serine cannot replace CDP-ethanolamine, N-acetyl-L-serine, L-homoserine, 4-hydroxy-L-proline, 5-hydroxy-DL-lysine, 3-hydroxy-DL-glutamic acid, L-threonine, ethanolamine or 3-hydroxypropionic acid cannot replace serine | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SEP synthetase | - |
Gallus gallus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 60 min | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 55% of maximal activity at pH 6.5, 30% of maximal activity at pH 9.0 | Gallus gallus |