Application | Comment | Organism |
---|---|---|
medicine | isoform PptT is a target for anti-tuberculosis drugs | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)DELTAentD cells | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mycobacterium tuberculosis | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Mycobacterium tuberculosis | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Mycobacterium tuberculosis BCG | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis BCG | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose affinity column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Mycobacterium tuberculosis BCG | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-phosphopantetheinyl transferase | - |
Mycobacterium tuberculosis |
PPTase | - |
Mycobacterium tuberculosis |
PptT | isoform | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheine | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | isoform PptT plays a role in the biosynthesis pathways for mycolic acids, polyketide-derived lipids and siderophores | Mycobacterium tuberculosis |
physiological function | the enzyme is required for Mycobacterium tuberculosis growth and persistence in vivo. The enzyme is involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors in mycobacteria. The enzyme is required for the replication and survival of the tubercle bacillus during the acute and chronic phases of infection in mice | Mycobacterium tuberculosis |