Cloned (Comment) | Organism |
---|---|
gene ppsA, overexpression in strain BL21 (DE3) | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | Escherichia coli | involved in gluconeogenesis | AMP + phosphoenolpyruvate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23538 | gene ppsA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from overexpression, purification in a single chromatographic step | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-fluoropyruvate + H2O | stereochemically specific reaction | Escherichia coli | AMP + (Z)-fluorophosphoenolpyruvate + phosphate | - |
r | |
ATP + 3-fluoropyruvate + H2O | 10times slower reaction rate than with pyruvate | Escherichia coli | AMP + (Z)-fluorophosphoenolpyruvate + phosphate | - |
r | |
ATP + pyruvate + H2O | - |
Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r | |
ATP + pyruvate + H2O | involved in gluconeogenesis | Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
phosphoenol pyruvate synthetase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
pyruvate formation | Escherichia coli |
8.4 | - |
phosphoenolpyruvate formation | Escherichia coli |